Ben Ali Gam Zouhaier, Thioye Abdoulaye, Cayol Jean-Luc, Joseph Manon, Fauque Guy, Labat Marc
Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0 UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
Laboratoire de Microbiologie Appliquée et de Génie Industriel, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop, BP 5005 Dakar-Fann, Dakar, Sénégal.
Int J Syst Evol Microbiol. 2018 Mar;68(3):715-720. doi: 10.1099/ijsem.0.002567. Epub 2018 Jan 12.
A novel slightly halophilic sulfate-reducing bacterium, designated strain P1BSR, was isolated from water of a saline lake in Tunisia. Strain P1BSR had motile (single polar flagellum), Gram-negative, rod-shaped, non-spore-forming cells, occurring singly or in pairs. Strain P1BSR grew at temperatures between 15 and 45 °C (optimum 40 °C), and in a pH range between 6 and 8.5 (optimum pH 6.7). The strain required NaCl for growth (1 % w/v), and tolerated high NaCl concentration (up to 12 % w/v) with an optimum of 3 % (w/v). Sulfate, thiosulfate and sulfite served as terminal electron acceptors, but not elemental sulfur, fumarate, nitrate and nitrite. Strain P1BSR utilized lactate, pyruvate, formate, d-fructose and glycerol as carbon and energy sources. The main cellular fatty acid was C16 : 0 (50.8 %). The genomic DNA G+C content was 47.7 mol%. Phylogenetic analysis of 16S rRNA gene sequence similarity indicated that strain P1BSR was affiliated to the genus Desulfovibrio, with the type strains Desulfovibrio salexigens (96.51 %), Desulfovibrio zosterae (95.68 %), Desulfovibrio hydrothermalis (94.81 %) and Desulfovibrio ferrireducens (94.73 %) as its closest phylogenetic relatives. On the basis of genotypic, phenotypic and phylogenetic characteristics, it is proposed to assign strain P1BSR to a novel species of the genus Desulfovibrio, Desulfovibrio salinus sp. nov. The type strain is P1BSR (=DSM 101510=JCM 31065).
从突尼斯一个盐湖的水中分离出一株新型轻度嗜盐硫酸盐还原菌，命名为菌株P1BSR。菌株P1BSR具有运动性（单极鞭毛），革兰氏阴性，杆状，不形成芽孢，细胞单个或成对出现。菌株P1BSR在15至45°C（最适温度40°C）的温度范围内生长，pH范围为6至8.5（最适pH 6.7）。该菌株生长需要NaCl（1% w/v），并能耐受高浓度NaCl（高达12% w/v），最适浓度为3%（w/v）。硫酸盐、硫代硫酸盐和亚硫酸盐作为末端电子受体，但元素硫、富马酸盐、硝酸盐和亚硝酸盐不能作为末端电子受体。菌株P1BSR利用乳酸、丙酮酸、甲酸、d-果糖和甘油作为碳源和能源。主要细胞脂肪酸为C16:0（50.8%）。基因组DNA G+C含量为47.7 mol%。基于16S rRNA基因序列相似性的系统发育分析表明，菌株P1BSR隶属于脱硫弧菌属，与盐生脱硫弧菌（96.51%）、大叶藻脱硫弧菌（95.68%）、热液脱硫弧菌（94.81%）和铁还原脱硫弧菌（94.73%）等模式菌株为最近的系统发育亲缘关系。基于基因型、表型和系统发育特征，建议将菌株P1BSR归为脱硫弧菌属的一个新物种，即盐生脱硫弧菌（Desulfovibrio salinus sp. nov.）。模式菌株为P1BSR（=DSM 101510=JCM 31065）。
