Radosavljević Ivan, Satovic Zlatko, Jakse Jernej, Javornik Branka, Greguraš Danijela, Jug-Dujaković Marija, Liber Zlatko
Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9A, 10000 Zagreb, Croatia.
Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia.
Int J Mol Sci. 2012;13(9):12082-12093. doi: 10.3390/ijms130912082. Epub 2012 Sep 24.
Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H(E)) was compared to the expected genetic diversity at mutation-drift equilibrium (H(EQ)) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.
从药用鼠尾草中分离出九个新的微卫星标记（SSR）。在亚得里亚海东岸的一个自然种群中检测到总共125个等位基因，每个位点有8至21个等位基因。观察到的杂合度、预期杂合度和多态信息含量分别为0.46至0.83、0.73至0.93和0.70至0.92。新的微卫星标记以及先前发表的标记在短齿鼠尾草（Salvia brachyodon Vandas）中进行了交叉扩增测试，短齿鼠尾草是一种狭域特有物种，仅在巴尔干半岛的两个地方有分布。在对短齿鼠尾草自然种群测试的30个微卫星标记中，有15个成功扩增。为了获得这两个物种种群近期瓶颈事件的证据，将观察到的遗传多样性（H(E)）与突变 - 漂变平衡时的预期遗传多样性（H(EQ)）进行比较，并使用两阶段突变模型（TPM）根据观察到的等位基因数量进行计算。仅在短齿鼠尾草种群中检测到近期瓶颈事件。这一结果表明有必要重新考虑该特有物种当前的受威胁类别。
