牡丹（Paeonia suffruticosa）EST-SSR 标记的开发与特性分析。

Development and characterization of EST-SSR markers in Paeonia suffruticosa (Paeoniaceae).

机构信息

College of Agriculture, Henan University of Science and Technology, Luoyang 471003, People's Republic of China.

出版信息

Am J Bot. 2011 Nov;98(11):e303-5. doi: 10.3732/ajb.1100172. Epub 2011 Oct 14.

DOI:10.3732/ajb.1100172

PMID:22003178

Abstract

PREMISE OF THE STUDY

Paeonia suffruticosa is one of the most famous flowering species in China. Ten simple sequence repeat (SSR) markers were developed and characterized from the expressed sequence tag (EST) database of tree peonies to be applied in genetic diversity studies.

METHODS AND RESULTS

A total of 10 novel EST-SSR loci were developed and characterized when tested on two populations of 45 tree peony individuals from China. The number of alleles per locus ranged from one to four and the expected heterozygosity ranged from 0.2087 to 0.6581. Select loci also amplified successfully in three other Paeonia species (P. ostii, P. rockii, P. jishanensis).

CONCLUSIONS

These markers will facilitate analysis of genetic diversity, population differentiation, and marker-assisted breeding of Paeoniaceae.

摘要

研究前提

牡丹是中国最著名的花卉物种之一。本研究从牡丹的表达序列标签（EST）数据库中开发并鉴定了 10 个简单序列重复（SSR）标记，以应用于遗传多样性研究。

方法和结果

在中国的两个牡丹群体 45 个个体中，共开发和鉴定了 10 个新的 EST-SSR 标记。每个标记的等位基因数从 1 到 4 不等，预期杂合度从 0.2087 到 0.6581 不等。选择的标记也能在其他三种芍药属植物（P. ostii、P. rockii 和 P. jishanensis）中成功扩增。