开发并鉴定 18 个海桑属 EST-SSR 标记。

Development and characterization of 18 EST-SSR markers in Sonneratia caseolaris.

机构信息

State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China.

出版信息

Am J Bot. 2011 Apr;98(4):e78-80. doi: 10.3732/ajb.1000433. Epub 2011 Apr 4.

DOI:10.3732/ajb.1000433

PMID:21613152

Abstract

PREMISE OF THE STUDY

Sonneratia caseolaris, a typical mangrove species, is widely distributed in the Indo-West Pacific region. EST-SSR markers were developed for this species to examine its genetic diversity.

METHODS AND RESULTS

A total of 18 EST-SSR primer pairs were designed based on the transcriptome sequences of S. caseolaris. Thirteen primer pairs showed polymorphism with one to three alleles per locus when assessed in two populations from China and Australia. The observed heterozygosity and expected heterozygosity ranged from 0 to 0.5000, and 0 to 0.5217 in the Hainan population, and from 0 to 0.2500, and 0 to 0.4891 in the Queensland population, respectively. Thirteen of the 18 primer sets identified in S. caseolaris can be successfully applied to its congener S. alba, and a much lower level of polymorphisms was observed in this widespread species.

CONCLUSIONS

These polymorphic EST-SSR markers for S. caseolaris are likely to be useful for future genetic diversity studies.

摘要

研究前提

海桑属（Sonneratia）的典型红树林物种海桑（S. caseolaris）广泛分布于印度洋-西太平洋地区。本研究旨在开发 EST-SSR 标记，以评估其遗传多样性。

方法与结果

本研究基于海桑转录组序列设计了 18 对 EST-SSR 引物，在中国和澳大利亚的两个种群中评估了其中 13 对引物，发现每个位点有 1-3 个等位基因具有多态性。在海南种群中，观察到的杂合度和期望杂合度范围分别为 0 至 0.5000 和 0 至 0.5217，在昆士兰种群中，范围分别为 0 至 0.2500 和 0 至 0.4891。在海桑属的 18 对引物中，有 13 对可成功应用于分布广泛的同属物种海白树（S. alba），并观察到该物种的多态性较低。