Department of Biochemistry, Faculty of Agriculture, Cairo University, Cairo, Egypt.
PLoS One. 2011;6(10):e25512. doi: 10.1371/journal.pone.0025512. Epub 2011 Oct 7.
The changes in phytate, phytase activity and in vitro bioavailability of iron and zinc during soaking and germination of three white sorghum varieties (Sorghum bicolor L. Moench), named Dorado, Shandweel-6, and Giza-15 were investigated. Sorghum varieties were soaked for 20 h and germinated for 72 h after soaking for 20 h to reduce phytate content and increase iron and zinc in vitro bioavailability. The results revealed that iron and zinc content was significantly reduced from 28.16 to 32.16% and 13.78 to 26.69% for soaking treatment and 38.43 to 39.18% and 21.80 to 31.27% for germination treatments, respectively. Phytate content was significantly reduced from 23.59 to 32.40% for soaking treatment and 24.92 to 35.27% for germination treatments, respectively. Phytase enzymes will be activated during drying in equal form in all varieties. The results proved that the main distinct point is the change of phytase activity as well as specific activity during different treatment which showed no significant differences between the varieties used. The in vitro bioavailability of iron and zinc were significantly improved as a result of soaking and germination treatments.
研究了三种白高粱品种（高粱 bicolor L. Moench），即 Dorado、Shandweel-6 和 Giza-15，在浸泡和发芽过程中植酸、植酸酶活性和铁锌体外生物利用度的变化。将高粱品种浸泡 20 小时，然后浸泡 20 小时后再发芽 72 小时，以降低植酸含量并提高铁和锌的体外生物利用度。结果表明，浸泡处理使铁和锌的含量分别从 28.16%降至 32.16%和从 13.78%降至 26.69%，发芽处理使铁和锌的含量分别从 38.43%降至 39.18%和从 21.80%降至 31.27%。植酸含量分别从 23.59%降至 32.40%和从 24.92%降至 35.27%，浸泡处理。在所有品种中，干燥过程中都会以相同的形式激活植酸酶。结果证明，主要的区别点是不同处理过程中植酸酶活性和比活的变化，所用品种之间没有显著差异。浸泡和发芽处理显著提高了铁和锌的体外生物利用度。
