Kitamura K, Yukawa T, Morita S, Ichiki Y, Eto T, Tanaka K
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):497-503. doi: 10.1016/0006-291x(90)92119-k.
In the present study a specific and sensitive radioimmunoassay for big endothelin-1 was developed. Half maximal inhibition of binding of radioiodinated big endothelin-1 was observed at 58 pg/tube and big endothelin-1 was detectable as low as 2 pg/tube. With this assay, the regional distribution of big endothelin-1 was determined in porcine tissue and compared to the distribution of an immunoreactive endothelin. Considerable amount of immunoreactive big endothelin-1 was observed in the aortic intima (0.84 +/- 0.094 pg/mg wet tissue; mean +/- S.D.) and the lung (0.47 +/- 0.055), but there was a low concentration in other tissue including the kidney inner medulla, which has been shown to be abundant in immunoreactive endothelin. Furthermore the molecular form of immunoreactive big endothelin-1 in aortic intima was found to be big ET-1[1-39], but the molecular form of major immunoreactive big endothelin-1 in the lung is big endothelin-1[22-39] with big endothelin-1[1-39] being minor.
在本研究中，开发了一种针对大内皮素-1的特异性和灵敏的放射免疫分析方法。在58 pg/管时观察到放射性碘化大内皮素-1结合的半数最大抑制，大内皮素-1的检测下限低至2 pg/管。采用该分析方法，测定了猪组织中大内皮素-1的区域分布，并与免疫反应性内皮素的分布进行了比较。在主动脉内膜（0.84±0.094 pg/毫克湿组织；平均值±标准差）和肺（0.47±0.055）中观察到相当数量的免疫反应性大内皮素-1，但在包括肾内髓质在内的其他组织中浓度较低，肾内髓质已被证明富含免疫反应性内皮素。此外，发现主动脉内膜中免疫反应性大内皮素-1的分子形式为大ET-1[1-39]，但肺中主要免疫反应性大内皮素-1的分子形式是大内皮素-1[22-39]，大内皮素-1[1-39]为次要形式。
