Kitamura K, Tanaka T, Kato J, Eto T, Tanaka K
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Life Sci. 1990;46(6):405-9. doi: 10.1016/0024-3205(90)90083-4.
Using a specific and sensitive radioimmunoassay for endothelin, combined with gel filtration and reverse phase high performance liquid chromatography, the molecular form of immunoreactive endothelin in the rat lung was investigated. On reverse phase high performance chromatography, the major immunoreactive endothelin in the rat lung emerged at a position identical to that of authentic endothelin-1. After oxidation of the immunoreactive endothelin by H2O2, the immunoreactivity migrated to a position identical to that of Met-sulfoxide endothelin-1. These data indicate that the major immunoreactive endothelin in the rat lung is not endothelin-3 (putative rat/human endothelin), but is identical or very similar to endothelin-1 (porcine/human endothelin).
采用针对内皮素的特异性灵敏放射免疫分析法，并结合凝胶过滤和反相高效液相色谱法，对大鼠肺中免疫反应性内皮素的分子形式进行了研究。在反相高效色谱上，大鼠肺中的主要免疫反应性内皮素出现的位置与天然内皮素-1相同。在用H2O2氧化免疫反应性内皮素后，免疫反应性迁移至与甲硫氨酸亚砜内皮素-1相同的位置。这些数据表明，大鼠肺中的主要免疫反应性内皮素不是内皮素-3（推测的大鼠/人内皮素），而是与内皮素-1（猪/人内皮素）相同或非常相似。
