Department of Food Science, University of Copenhagen , Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
J Agric Food Chem. 2011 Nov 23;59(22):11895-902. doi: 10.1021/jf201822p. Epub 2011 Nov 1.
Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN(-) and rn(+) genotype) were analyzed by both (1)H liquid-state NMR spectroscopy and a biochemical method. The (1)H NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of α-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different post-mortem glycogen degradation mechanisms in the two genotypes.
采用（1）H 液体核磁共振波谱法和生化法分析了携带和不携带 PRKAG3 基因突变（RN（-）和 rn（+）基因型）的肌间沟肉提取物中的酸溶性糖原（大糖原）。（1）H NMR 分析表明，死后 24-48 小时内生成了较短的α-1,4 连接葡萄糖聚合物（二聚体、三聚体等）。这是生化方法无法阐明的，生化方法只能测定水解葡萄糖残基的总量。较短的聚合物主要在 PRKAG3 基因突变的携带者中形成，这表明两种基因型的死后糖原降解机制不同。
