Department of Food Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
J Agric Food Chem. 2011 Nov 9;59(21):11608-15. doi: 10.1021/jf201936h. Epub 2011 Oct 14.
Postmortem changes in pork muscle protein phosphorylation in relation to the RN(-) genotype were investigated using one-dimensional gel electrophoresis and a phosphor specific staining. The phosphorylation levels of several protein bands were found to be affected by the RN(-) genotype and to change during postmortem development. Glycogen phosphorylase, phosphofructokinase, and pyruvate kinase were found in protein bands affected by the RN(-) genotype, and the phosphorylation profile indicates that part of the increased rate and extended pH decline of the RN(-) genotype could be a consequence of phosphorylation of these key enzymes during the postmortem metabolism. The results illustrate that the protein phosphorylation level of the muscle proteins could be interpreted as a global metabolic fingerprint containing information about the activity status of the enzymes in the postmortem metabolism.
采用一维凝胶电泳和磷特异性染色研究了与 RN(-)基因型相关的猪肉肌肉蛋白磷酸化的死后变化。发现几个蛋白条带的磷酸化水平受 RN(-)基因型的影响，并在死后发育过程中发生变化。糖原磷酸化酶、磷酸果糖激酶和丙酮酸激酶存在于受 RN(-)基因型影响的蛋白条带中，磷酸化图谱表明，RN(-)基因型的速率增加和 pH 值下降延长的部分原因可能是这些关键酶在死后代谢过程中的磷酸化。结果表明，肌肉蛋白的蛋白磷酸化水平可以解释为包含有关死后代谢中酶活性状态信息的全局代谢指纹。
