Department of Animal Science, Viçosa Federal University, Av. P.H. Rolfs s/n, Viçosa, MG, Brazil.
Anim Reprod Sci. 2011 Nov;129(1-2):50-5. doi: 10.1016/j.anireprosci.2011.09.012. Epub 2011 Oct 1.
Intravaginal progesterone devices are used worldwide for estrus induction in goats. Reused devices are able to induce estrus; however, this can be a health risk within a flock. The objective was to compare new and previously used (and autoclaved) progesterone-releasing intravaginal devices for induction of estrus and ovulation in seasonally anestrous Toggenburg goats. Anestrous goats (n=42) received new intravaginal devices containing 0.3g progesterone (CONTROL), or similar devices previously used for either 6 (USED6) or 12d (USED12) and subsequently autoclaved. All goats received 5mg dinoprost at device insertion and 200 IU eCG 5d later, and all devices were removed after 6d. After device removal, estrus was monitored and females displaying signs of estrus were mated by fertile bucks. Transrectal ovarian ultrasonography was performed after device removal until detection of ovulation. Blood samples were collected for determination of plasma progesterone concentration at different times. There was no difference (P>0.05) among groups CONTROL, USED6 or USED12 for: estrus response (87, 100 or 100%, respectively); duration of estrus (32.3±2.3, 25.2±3.4 or 27.3±4.1h); ovulation rate (100, 88 or 100%); number of ovulations (1.5±0.2, 1.9±0.3 or 1.7±0.3); and pregnancy rate (60, 58 or 67%). Plasma progesterone (P4) concentrations were greater (P<0.05) in CONTROL than in USED6-treated and USED12-treated goats (7.2±1.2, 4.7±0.7 and 4.3±0.6 ng/mL, respectively) at 6h after device insertion; these differences were maintained until 4d after device insertion (3.4±0.4, 2.3±0.2, and 2.5±0.2 ng/mL). Overall, plasma progesterone concentrations were greater (P<0.05) in nulliparous than in lactating goats (3.1±0.8 compared to 2.4±0.6 ng/mL, respectively). In conclusion, autoclaved, previously used intravaginal progesterone-releasing devices resulted in significant lesser plasma progesterone concentrations than new devices, but were similarly effective in inducing estrus and ovulation in anestrous goats.
阴道内孕酮释放装置在全球范围内用于诱导山羊发情。重复使用的装置能够诱导发情；然而，这在羊群中可能是一个健康风险。本研究的目的是比较新的和以前使用过的（并经过高压灭菌）孕酮释放阴道内装置，以诱导季节性乏情的托根堡山羊发情和排卵。乏情的山羊（n=42）接受含有 0.3g 孕酮的新阴道内装置（CONTROL），或以前使用过 6 天（USED6）或 12 天（USED12）并随后进行高压灭菌的类似装置。所有山羊在装置插入时接受 5mg 地诺前列酮，并在 5 天后接受 200IU eCG，所有装置在 6 天后取出。装置取出后，监测发情，发情表现的雌性与有生育能力的公山羊交配。在装置取出后，通过直肠超声检查卵巢，直到检测到排卵。在不同时间采集血液样本以测定血浆孕酮浓度。在 CONTROL、USED6 或 USED12 组之间，发情反应（分别为 87%、100%和 100%）；发情持续时间（32.3±2.3、25.2±3.4 或 27.3±4.1h）；排卵率（100%、88%或 100%）；排卵数（1.5±0.2、1.9±0.3 或 1.7±0.3）；和妊娠率（60%、58%或 67%）无差异（P>0.05）。在装置插入后 6 小时，CONTROL 组的血浆孕酮（P4）浓度高于 USED6 处理组和 USED12 处理组（7.2±1.2、4.7±0.7 和 4.3±0.6ng/mL）；这些差异一直持续到装置插入后 4 天（3.4±0.4、2.3±0.2 和 2.5±0.2ng/mL）。总体而言，初产母羊的血浆孕酮浓度高于哺乳期母羊（3.1±0.8 与 2.4±0.6ng/mL）。综上所述，经过高压灭菌的、以前使用过的阴道内孕酮释放装置导致的血浆孕酮浓度明显低于新装置，但在诱导乏情山羊发情和排卵方面同样有效。
