State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Food Chem Toxicol. 2011 Dec;49(12):3046-54. doi: 10.1016/j.fct.2011.10.001. Epub 2011 Oct 7.
Tubeimoside-1 is a triterpenoid saponin extracted from the traditional Chinese herb Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae). We investigated the cytotoxic effect and apoptosis mechanism of tubeimoside-1. Tubeimoside-1 was cytotoxic in seven human cancer cell lines, with HepG2 the most sensitive. Tubeimoside-1 induced apoptosis of HepG2 cells dose and time dependently. Both the extrinsic and intrinsic pathways were triggered by tubeimoside-1. Caspase-3, -8 and -9 were activated and the expression of Fas, Fas ligand, Bcl-2, Bak and Bax was regulated. Moreover, tubeimoside-1 induced accumulation of reactive oxygen species and arrested cell cycle at the G(2)/M phase, thus contributing to apoptosis, through signaling regulation by tumor necrosis factor α, nuclear factor κB (NF-κB), Jun N-terminal kinase (JNK) and p53. We provide further insight into the tubeimoside-1 cytotoxic effect for antitumor chemotherapeutic treatment.
葫芦素 -1 是一种从传统中药苦葫芦（葫芦科）中提取的三萜皂苷。我们研究了葫芦素 -1 的细胞毒性作用和凋亡机制。葫芦素 -1 在七种人类癌细胞系中具有细胞毒性，其中 HepG2 最为敏感。葫芦素 -1 诱导 HepG2 细胞凋亡呈剂量和时间依赖性。两种外在和内在途径均被葫芦素 -1 触发。半胱天冬酶 -3、-8 和 -9 被激活，Fas、Fas 配体、Bcl-2、Bak 和 Bax 的表达受到调节。此外，葫芦素 -1 通过肿瘤坏死因子 α、核因子 κB（NF-κB）、Jun N-末端激酶（JNK）和 p53 的信号转导调节，诱导活性氧的积累，并将细胞周期阻滞在 G(2)/M 期，从而促进凋亡。我们为抗肿瘤化疗治疗提供了进一步深入了解葫芦素 -1 的细胞毒性作用。
