Department of Geriatrics, The Shengjing Hospital of China Medical University, Liaoning 110004, PR China.
Mol Med Rep. 2011 Jan-Feb;4(1):25-9. doi: 10.3892/mmr.2010.379. Epub 2010 Oct 11.
Plant materials are a great source of cancer chemotherapeutic agents. Tubeimoside-1 (TBMS1) is a triterpenoid saponin extracted from the tubers of Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae), a widely prescribed traditional Chinese medicinal plant that is used for its antiviral and anti-inflammatory properties. The present study was conducted to investigate the effects of TBMS1 on the growth and apoptosis of human lung cancer A549 cells. TBMS1 inhibited the proliferation of the cells in a dose- and time-dependent manner. Apoptosis was confirmed by flow cytometry, fluorescence microscopy and transmission electron microscopy. TBMS1 treatment induced apoptosis by decreasing the expression of Bcl-2 and increasing the expression of Bax in the A549 cells. Inhibition of COX-2 was also observed after treating the cells with TBMS1. TBMS1 may prove to be a useful approach for the chemotherapy of lung cancer.
植物材料是癌症化疗药物的重要来源。 从葫芦科（Cucurbitaceae）植物苦葫芦（Bolbostemma paniculatum（Maxim.）Franquet）的块茎中提取的三萜皂苷 1（TBMS1）是一种具有抗病毒和抗炎特性的广泛应用的传统中药。 本研究旨在探讨 TBMS1 对人肺癌 A549 细胞生长和凋亡的影响。 TBMS1 以剂量和时间依赖的方式抑制细胞的增殖。 通过流式细胞术，荧光显微镜和透射电子显微镜证实了细胞凋亡。 TBMS1 通过降低 A549 细胞中 Bcl-2 的表达并增加 Bax 的表达诱导细胞凋亡。 在用 TBMS1 处理细胞后，还观察到 COX-2 的抑制。 TBMS1 可能成为治疗肺癌的一种有用方法。
