Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
Comp Immunol Microbiol Infect Dis. 2011 Dec;34(6):503-12. doi: 10.1016/j.cimid.2011.09.003. Epub 2011 Oct 17.
The present study was focused on the modulation of innate immune response genes in CNS of mouse in response to rabies virus (RABV) infection. The global gene expression changes in brains of RABV- or mock-infected mice were investigated using DNA microarray analysis and quantitative real-time PCR. Then functional enrichment of the differentially expressed mRNAs was performed. Microarray analysis showed that 390 genes in brain were significantly (P<0.01) regulated in response to RABV infection, with obviously up-regulated genes like interferon (IFN) stimulated genes (ISGs), IFN inducible transcription factors, cytokines and complement, etc. The significant pathways of differentially expressed genes are mainly involved in JAK-STAT signaling pathway, antigen processing and presentation, ubiquitin mediated proteolysis and complement cascades. The results suggest that the modulated genes in infected CNS were possibly involved in pathogenesis of rabies. Conversely, they may have protective effects.
本研究专注于研究狂犬病病毒（RABV）感染后小鼠中枢神经系统固有免疫反应基因的调控。采用 DNA 微阵列分析和实时定量 PCR 方法研究了 RABV 或模拟感染小鼠大脑中的全基因表达变化。然后对差异表达的 mRNA 进行了功能富集分析。微阵列分析显示，390 个基因在大脑中因 RABV 感染而受到明显调控（P<0.01），明显上调的基因如干扰素（IFN）刺激基因（ISGs）、IFN 诱导转录因子、细胞因子和补体等。差异表达基因的显著通路主要涉及 JAK-STAT 信号通路、抗原加工和呈递、泛素介导的蛋白水解和补体级联。结果表明，感染中枢神经系统中调节的基因可能参与狂犬病的发病机制。相反，它们可能具有保护作用。
