Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, PR China.
Microb Pathog. 2012 Jan;52(1):47-54. doi: 10.1016/j.micpath.2011.10.001. Epub 2011 Oct 12.
MicroRNAs (miRNAs) are small RNA (≈ 22 nt) molecules expressed endogenously in cells. They are involved in the regulation of gene expression. Recently, evidence has shown that cellular miRNAs have key regulatory roles in virus-host interactions. The rabies virus (RABV) causes a fatal infection of the central nervous systems (CNS) of warm-blooded animals, yet its pathogenesis remains poorly understood. To gain more insight into the pathogenesis of RABV, a miRNA microarray was performed as part of an investigation of changes in host miRNA expression in the brains of mice infected with RABV. The results showed that RABV infection induced modulation of the expression of sixteen miRNA molecules. These data were verified by real-time PCR. Functional analysis showed the differentially expressed miRNAs to be involved in many immune-related signaling pathways, such as the RIG-I-like receptor signaling pathway, JAK-STAT signaling pathway, chemokine signaling pathway, T-cell receptor signaling pathway, MAPK signaling pathway, leukocyte transendothelial migration, and natural killer cell mediated cytotoxicity. The predicted expression levels of the target genes of these modulated miRNAs correlated with measurements of gene expression measured by DNA microarray and qRT-PCR.
MicroRNAs (miRNAs) 是细胞内表达的内源性小 RNA（≈22nt）分子。它们参与基因表达的调控。最近的证据表明，细胞 miRNAs 在病毒-宿主相互作用中具有关键的调节作用。狂犬病病毒（RABV）会导致温血动物中枢神经系统（CNS）的致命感染，但它的发病机制仍不清楚。为了更深入地了解 RABV 的发病机制，作为对感染 RABV 的小鼠大脑中宿主 miRNA 表达变化的研究的一部分，进行了 miRNA 微阵列分析。结果表明，RABV 感染诱导了十六种 miRNA 分子表达的调节。这些数据通过实时 PCR 得到了验证。功能分析表明，差异表达的 miRNAs 参与了许多免疫相关的信号通路，如 RIG-I 样受体信号通路、JAK-STAT 信号通路、趋化因子信号通路、T 细胞受体信号通路、MAPK 信号通路、白细胞跨内皮迁移和自然杀伤细胞介导的细胞毒性。这些调节 miRNA 的靶基因的预测表达水平与 DNA 微阵列和 qRT-PCR 测量的基因表达测量值相关。
