Department of Horticulture, Biotechnology and Landscape Architecture, Plant Virus GenBank, Seoul Women's University, Seoul 139-774, Korea.
Arch Virol. 2012 Jan;157(1):185-7. doi: 10.1007/s00705-011-1142-5. Epub 2011 Oct 18.
In this study, we identified a new tobamovirus from diseased Aporcactus flagelliformis cactus plants, named it rattail cactus necrosis-associated virus (RCNaV), and determined its complete genome sequence. The full RCNaV genome consisted of 6,506 nucleotides and contained four open reading frames coding for proteins of M(r) 128 kDa (3,441 nt), 185 kDa (4,929 nt), 55 kDa (1452 nt), 36 kDa (1,005 nt) and 19 kDa (513 nt) from the 5' to 3' end, respectively. The overall similarities for the four ORFs of RCNaV were from 32.5% to 64.1% and from 17.0% to 67.3% to those of the other tobamoviruses, at the nucleotide and amino acid level, respectively. Comparison of the coding and non-coding regions of the virus with those of other tobamoviruses showed that RCNaV is the most closely related to cactus mild mottle virus.
在这项研究中，我们从患病的 Aporcactus flagelliformis 仙人掌植物中鉴定出一种新的烟草花叶病毒，将其命名为响尾蛇仙人掌坏死相关病毒（RCNaV），并确定了其完整的基因组序列。RCNaV 的全基因组由 6506 个核苷酸组成，包含四个开放阅读框，分别编码分子量为 128kDa（3441nt）、185kDa（4929nt）、55kDa（1452nt）、36kDa（1005nt）和 19kDa（513nt）的蛋白。RCNaV 的四个 ORF 从 5'到 3'端的核苷酸和氨基酸水平上与其他烟草花叶病毒的相似性分别为 32.5%至 64.1%和 17.0%至 67.3%。对病毒的编码和非编码区与其他烟草花叶病毒的比较表明，RCNaV 与仙人掌轻度斑驳病毒最为密切相关。
