Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul 139-774, Korea.
Arch Virol. 2011 Jun;156(6):1093-5. doi: 10.1007/s00705-011-0961-8. Epub 2011 Mar 9.
In this study, we determined the complete sequence of the genomic RNA of a Florida isolate of maracuja mosaic virus (MarMV-FL) and compared it to that of a Peru isolate of the virus (MarMV-P) and those of other known tobamoviruses. Complete sequence analysis revealed that the isolate should be considered a member of a new species and named passion fruit mosaic virus (PafMV). The genomic RNA of PafMV consists of 6,791 nucleotides and encodes four open reading frames (ORFs) coding for proteins of 125 kDa (1,101 aa), 184 kDa (1,612 aa), 34 kDa (311 aa) and 18 kDa (164 aa) in consecutive order from the 5' to the 3' end. The sequence homologies of the four ORFs of PafMV were from 78.8% to 81.6% to those of MarMV-P at the amino acid level. The sequence homologies of the four ORFs of PafMV ranged from 36.0% to 77.9% and from 21.7% to 81.6% to those of other tobamoviruses, at the nucleotide and amino acid level, respectively. Phylogenetic analysis revealed that these PafMV-encoded proteins are closely related to those of MarMV-P. In conclusion, the results indicate that PafMV and MarMV-P belong to different species within the genus Tobamovirus.
在本研究中，我们确定了佛罗里达分离株马拉瓜花叶病毒（MarMV-FL）基因组 RNA 的完整序列，并将其与病毒的秘鲁分离株（MarMV-P）以及其他已知的烟草花叶病毒进行了比较。完整序列分析表明，该分离株应被视为一个新种的成员，并命名为百香果花叶病毒（PafMV）。PafMV 的基因组 RNA 由 6791 个核苷酸组成，编码四个开放阅读框（ORF），分别编码 125 kDa（1101 个氨基酸）、184 kDa（1612 个氨基酸）、34 kDa（311 个氨基酸）和 18 kDa（164 个氨基酸）的蛋白。PafMV 的四个 ORF 的序列同源性在氨基酸水平上与 MarMV-P 的同源性为 78.8%至 81.6%。PafMV 的四个 ORF 的序列同源性在核苷酸和氨基酸水平上分别为 36.0%至 77.9%和 21.7%至 81.6%，与其他烟草花叶病毒。系统发育分析表明，这些 PafMV 编码的蛋白与 MarMV-P 编码的蛋白密切相关。总之，结果表明 PafMV 和 MarMV-P 属于烟草花叶病毒属中的不同种。
