Munson L, Wilkinson J E, Schlafer D H
Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Cell Tissue Res. 1990 Jul;261(1):155-61. doi: 10.1007/BF00329448.
Epithelial-cell function requires cellular polarity in which apical membrane surfaces have unique characteristics and cellular organelles are stratified. Physiological investigations of endometrial epithelial cells would be enhanced greatly by the ability of a method to polarize cells in culture. This study investigates the effects of different substrata on polarization of cultured bovine endometrial epithelial cells. Fetal bovine endometrial epithelial-cell lines were developed from explant outgrowth. Epithelial monolayers were subcultured onto amniotic membranes, Millicell-HA membranes, or Millicell-CM membranes coated with rat-tail collagen, Matrigel, laminin, Vitrogen, or fibronectin. Cultures on these substrata were maintained at the air/liquid interface. Cells grown on either collagen-coated or uncoated Millicell membranes also were maintained submerged in medium. Excellent polarized morphology was attained in cultures grown at the air/liquid interface on amniotic membranes and rat-tail collagen-coated membranes. Lectin-binding patterns to apical membranes of polarized epithelial cell cultures paralleled patterns of binding to bovine endometrial surfaces in vivo. Cultures on rat-tail collagen were maintained for several weeks. These methods provide a valuable system for studying the endometrium in vitro.
上皮细胞功能需要细胞极性，其中顶端膜表面具有独特特征且细胞器分层。如果有一种方法能够使培养的细胞极化，那么对子宫内膜上皮细胞的生理学研究将得到极大加强。本研究调查了不同基质对培养的牛子宫内膜上皮细胞极化的影响。从外植体生长物中建立了胎牛子宫内膜上皮细胞系。将上皮单层细胞传代培养到涂有大鼠尾胶原、基质胶、层粘连蛋白、维特克胶原或纤连蛋白的羊膜、密理博-HA膜或密理博-CM膜上。这些基质上的培养物保持在气液界面。在胶原包被或未包被的密理博膜上生长的细胞也保持浸没在培养基中。在羊膜和气液界面生长的大鼠尾胶原包被膜上的培养物中获得了极佳的极化形态。凝集素与极化上皮细胞培养物顶端膜的结合模式与体内与牛子宫内膜表面的结合模式相似。大鼠尾胶原上的培养物维持了数周。这些方法为体外研究子宫内膜提供了一个有价值的系统。
