Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.
Rapid Commun Mass Spectrom. 2011 Nov 15;25(21):3235-44. doi: 10.1002/rcm.5217.
Mono- or oligosaccharide-containing samples, whether they are derived from biological sources or products of chemical synthesis, are often mixtures of spatial or constitutional isomers. The possibility of characterising or performing quality control on such samples by mass spectrometry is hampered because these isomers cannot be separated by their mass-to-charge ratio alone. Therefore, the use of techniques to separate the isobaric sample compounds prior to mass spectrometric characterisation is mandatory. Travelling wave ion mobility separation offers the possibility of separating mixtures based on their compound's collisional cross-sections in the gas phase and can easily be combined with mass spectrometry for further characterisation. Here, we use 5-N-acetylneuraminic acid and several derivatives as model compounds to evaluate the separation power of travelling wave ion mobility spectrometry and present an approach to clearly identify constitutional isomers in mixtures in combination with low-energy collision-induced dissociation (CID) in the negative ion mode even if they cannot be completely separated by ion mobility.
单糖或寡糖含量的样本，无论是来源于生物来源还是化学合成产物，通常都是空间或构象异构体的混合物。通过质谱法对这些样本进行特征描述或质量控制的可能性受到阻碍，因为仅通过质荷比不能将这些异构体分开。因此，在进行质谱特征描述之前，必须使用分离等质量比样品化合物的技术。行波离子淌度分离提供了基于混合物在气相中的碰撞截面分离混合物的可能性，并且可以很容易地与质谱结合进行进一步的特征描述。在这里，我们使用 5-N-乙酰神经氨酸和几种衍生物作为模型化合物来评估行波离子淌度谱的分离能力，并提出了一种方法，即使在不能完全通过离子淌度分离的情况下，也可以结合负离子模式下的低能碰撞诱导解离（CID），清晰地识别混合物中的构象异构体。
