Yamaguchi Kei, Inoue Mari, Goshima Naoki
Department of Research and Development, Japan Biological Informatics Consortium (JBiC), 2-4-32 Aomi, Koto-Ku, Tokyo 135-8073, Japan.
J Biomed Biotechnol. 2011;2011:872065. doi: 10.1155/2011/872065. Epub 2011 Oct 9.
Cationic peptides termed protein transduction domains (PTDs) have been shown to cross biological membranes efficiently. However, proteins transduced by PTDs become entrapped within the endosomal vesicles and are not delivered into organelles. We have developed a novel protein delivery system to enhance the proton sponge effect, which results in rupture of the endosomes, by using a mixture of Wr-T transporter peptide and a commercially available cationic lipid reagent. This peptide and cationic lipid reagent mixture efficiently delivers a variety of cargo proteins into living cells by releasing them from the endosomes.
被称为蛋白质转导结构域（PTDs）的阳离子肽已被证明能有效穿过生物膜。然而，由PTDs转导的蛋白质会被困在内体小泡中，无法被递送至细胞器。我们开发了一种新型蛋白质递送系统，通过使用Wr-T转运肽和市售阳离子脂质试剂的混合物来增强质子海绵效应，从而导致内体破裂。这种肽与阳离子脂质试剂的混合物通过将各种货物蛋白从内体中释放出来，有效地将它们递送至活细胞中。
