不同材料修复后牙周膜成纤维细胞对牙根穿孔的反应：一项实验室研究。

Periodontal ligament fibroblast response to root perforations restored with different materials: a laboratory study.

机构信息

Department of Periodontology, Selcuk University, Konya.

出版信息

Int Endod J. 2012 Mar;45(3):240-8. doi: 10.1111/j.1365-2591.2011.01968.x. Epub 2011 Oct 19.

DOI:10.1111/j.1365-2591.2011.01968.x

PMID:22007644

Abstract

AIM

To compare the effect of several materials on the attachment of periodontal ligament (PDL) fibroblasts to experimentally perforated root surfaces.

METHODOLOGY

Root specimens (size 5 × 5 mm) were obtained from extracted human molar teeth and perforations with a 1 mm diameter were created. One group was kept as a control and the rest were repaired with the following materials: Amalgam, Dyract, IRM, Super Bond C&B and Mineral trioxide aggregate (MTA). PDL fibroblasts were placed at a density of 8 × 10(4) cells on the root specimens, incubated on tissue culture inserts (48 h) and then transferred to 48 well-plates. MTT assays were performed at 48 and 96 h for PDL fibroblast survival. Cell attachment was observed using confocal microscopy on days 2 and 5. Total RNAs from the root specimens were isolated on day 5 and type I collagen (COL I) and Runt-related transcription factor 2 (Runx2) mRNA expressions were checked using Quantitative-Polymerase Chain Reaction (QPCR). For the MTT assay and QPCR, one-way analysis of variance (anova) and Tukey HSD multiple comparison tests were used to compare the groups.

RESULTS

Mineral trioxide aggregate resulted in a significantly higher cell density (P < 0.001). Dyract, IRM and Super Bond C&B groups had a lower cell density when compared with the control and MTA groups at 48 h (P < 0.001). Confocal microscopy revealed that, among the experimental groups, the MTA group had the largest viable cell population over the restoration site when compared with the other materials; however, reduced cell attachment was noted in all groups when compared with the control. Increased Runx2 mRNA expressions were noted in MTA (P < 0.001) and IRM (P < 0.01) groups when compared with control and other tested materials. COL I transcripts were increased in IRM (P < 0.01), D, C&B and MTA (P < 0.001) when compared with the control.

CONCLUSION

Mineral trioxide aggregate provided a more favorable environment for PDL cell adhesion and growth.

摘要

目的

比较几种材料对牙周膜（PDL）成纤维细胞附着于实验性穿孔根面的影响。

方法

从拔除的人磨牙中获得大小为 5×5mm 的根标本，并制作直径为 1mm 的穿孔。一组作为对照，其余组用以下材料修复：银汞合金、Dyract、IRM、Super Bond C&B 和矿物三氧化物聚合体（MTA）。将 PDL 成纤维细胞以 8×10（4）个细胞/根标本的密度放置在根标本上，在组织培养插入物上孵育（48 小时），然后转移到 48 孔板中。在第 48 小时和第 96 小时进行 MTT 测定以评估 PDL 成纤维细胞的存活率。在第 2 天和第 5 天使用共聚焦显微镜观察细胞附着。在第 5 天从根标本中分离总 RNA，并使用定量聚合酶链反应（QPCR）检查 I 型胶原（COL I）和 runt 相关转录因子 2（Runx2）mRNA 的表达。对于 MTT 测定和 QPCR，使用单因素方差分析（anova）和 Tukey HSD 多重比较检验来比较各组。

结果

矿物三氧化物聚合体导致细胞密度显著增加（P < 0.001）。与对照组和 MTA 组相比，Dyract、IRM 和 Super Bond C&B 组在第 48 小时的细胞密度较低（P < 0.001）。共聚焦显微镜显示，在实验组中，与其他材料相比，MTA 组在修复部位的存活细胞数量最大；然而，与对照组相比，所有组的细胞附着均减少。与对照组和其他测试材料相比，MTA（P < 0.001）和 IRM（P < 0.01）组的 Runx2 mRNA 表达增加。与对照组相比，IRM（P < 0.01）、D、C&B 和 MTA（P < 0.001）组的 COL I 转录物增加。