人类小脑浦肯野细胞的细胞分选研究：男性和女性大脑左右半球中的细胞数量。
Fractionator studies on Purkinje cells in the human cerebellum: numbers in right and left halves of male and female brains.
作者信息
Mayhew T M, MacLaren R, Henery C C
机构信息
Department of Anatomy, Marischal College, University of Aberdeen, Scotland.
出版信息
J Anat. 1990 Apr;169:63-70.
Direct estimates of the numbers of Purkinje cell nucleoli in 22 aged human cerebella of known weight were obtained from paraffin-embedded sections using the fractionator. The nucleoli were counted in single sections. The estimates are unbiased by fixation, section thickness or sampling errors and are free from any assumptions about shape, size or spatial orientation. Left and right sides of the cerebellum were analysed in ten subjects whilst sex differences were examined in eleven subjects. No significant lateral differences in fixed weight or nucleolar number were observed. The group means (coefficients of variation) per side were 45 g (18%) and 7.8 millions (26%) respectively. The average complement of nucleoli in 22 cerebella amounted to 15.6 millions (22%). Estimated numbers did not show any significant correlation with cerebellar weights and, in a subsample of these brains (five male and six female), apparent sex differences were not significant.
使用分样器从石蜡包埋切片中直接估计22个已知重量的老年人类小脑浦肯野细胞核仁数量。在单张切片中对核仁进行计数。这些估计不受固定、切片厚度或抽样误差的影响，且无需对形状、大小或空间取向做任何假设。对10名受试者的小脑左右两侧进行了分析，同时对11名受试者的性别差异进行了研究。未观察到固定重量或核仁数量的显著侧别差异。每侧的组均值（变异系数）分别为45克（18%）和780万个（26%）。22个小脑的核仁平均总数为1560万个（22%）。估计数量与小脑重量无显著相关性，并且在这些大脑的一个子样本（5名男性和6名女性）中，明显的性别差异并不显著。
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