Braendgaard H, Gundersen H J
J Neurosci Methods. 1986 Oct;18(1-2):39-78. doi: 10.1016/0165-0270(86)90112-3.
The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for unbiased estimation of anisotropic surfaces. The examples include descriptions of estimators of neurone and synapse number and sizes, synapse gradients, neurone point patterns in three-dimensional space, capillary surface area, and perikarya volumes. A major advantage of the methods is the possibility to carry out the estimation procedures in specified, well-characterized regions or layers of the brain. Some general statistical and stereological problems are briefly discussed.
本文展示了一些新的体视学原理在无偏估计颗粒数量、大小及颗粒采样方面的实用性，同时还介绍了一种用于无偏估计各向异性表面的新原理。示例包括对神经元和突触数量、大小、突触梯度、三维空间中神经元点模式、毛细血管表面积以及核周体体积估计器的描述。这些方法的一个主要优点是能够在大脑特定的、特征明确的区域或层中进行估计程序。文中还简要讨论了一些一般的统计和体视学问题。
