Ji H, Lu R-H, Chang Z-G, Su S-S, Yang G-S
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Reprod Domest Anim. 2012 Jun;47(3):419-27. doi: 10.1111/j.1439-0531.2011.01894.x. Epub 2011 Oct 18.
This study determined the expression of genes involved in mitochondrial function and adipogenesis at mRNA and protein levels by transfecting rat differentiating preadipocytes with siRNA/Lipofectamine complex and pcDNA-PGC-1β (peroxisome proliferator-activated receptor-γ coactivator-1β)/Lipofectamine complex, respectively, to further elucidate the role of PGC-1β in white preadipocyte differentiation. The results showed that the transfection of PGC-1β siRNA inhibited the expressions of mitochondrial genes malate dehydrogenase, carnitine palmitoyltransferase 1, nuclear respiratory factor 1, ATP synthesis, adipocyte differentiation key transcription factor peroxisome proliferator-activated receptor-γ, sterol regulatory element binding protein 1c and fatty acid synthetase, whereas the triglyceride synthesis was retarded (p < 0.05). Furthermore, overexpression of PGC-1β up-regulated the expressions of adipogenic and mitochondrial biosynthetic marker genes and promoted triglyceride accumulation during 3T3-L1 adipocyte differentiation. These observations suggest that PGC-1β modulates the expression of mitochondrial function and adipogenesis-related genes and affects white preadipocyte differentiation.
本研究通过分别用小干扰RNA（siRNA）/脂质体复合物和质粒DNA（pcDNA）-过氧化物酶体增殖物激活受体γ共激活因子1β（PGC-1β）/脂质体复合物转染大鼠分化前脂肪细胞，在mRNA和蛋白质水平上确定参与线粒体功能和脂肪生成的基因表达，以进一步阐明PGC-1β在白色前脂肪细胞分化中的作用。结果显示，转染PGC-1β的小干扰RNA抑制了线粒体基因苹果酸脱氢酶、肉碱棕榈酰转移酶1、核呼吸因子1、ATP合成、脂肪细胞分化关键转录因子过氧化物酶体增殖物激活受体γ、固醇调节元件结合蛋白1c和脂肪酸合成酶的表达，而甘油三酯合成受到抑制（p<0.05）。此外，PGC-1β的过表达上调了脂肪生成和线粒体生物合成标记基因的表达，并促进了在3T3-L1脂肪细胞分化过程中的甘油三酯积累。这些观察结果表明，PGC-1β调节线粒体功能和脂肪生成相关基因的表达，并影响白色前脂肪细胞的分化。
