Hua Rong-Hong, Bu Zhi-Gao
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P.R. China.
Hybridoma (Larchmt). 2011 Oct;30(5):451-6. doi: 10.1089/hyb.2011.0027.
Japanese encephalitis virus (JEV) is a major public health threat in the Asia-Pacific region. The pre-membrane (PrM) protein of Japanese encephalitis virus is cleaved during maturation by the cellular protease into the structural protein M and a pr-segment. Here, we describe a procedure to generate monoclonal antibody (MAb) against JEV PrM/M protein and investigate its characteristics. Western blot analysis showed that the MAbs produced in this study were against JEV PrM/M specifically. Indirect immunofluorescence assay demonstrated that they could recognize native PrM/M protein in JEV-infected BHK-21 cells. Preliminary studies identified the epitope of the MAb with a set of synthesized overlapping peptides covering the whole length of PrM protein of JEV. The MAbs reported here may provide valuable tools for the further exploration of biological properties and functions of PrM/M protein and may also be developed for potential clinical applications.
日本脑炎病毒（JEV）是亚太地区主要的公共卫生威胁。日本脑炎病毒的前体膜（PrM）蛋白在成熟过程中被细胞蛋白酶切割成结构蛋白M和一个pr片段。在此，我们描述了一种产生针对JEV PrM/M蛋白的单克隆抗体（MAb）并研究其特性的方法。蛋白质免疫印迹分析表明，本研究中产生的单克隆抗体特异性针对JEV PrM/M。间接免疫荧光试验证明它们能够识别JEV感染的BHK - 21细胞中的天然PrM/M蛋白。初步研究用一组覆盖JEV PrM蛋白全长的合成重叠肽确定了单克隆抗体的表位。本文报道的单克隆抗体可能为进一步探索PrM/M蛋白的生物学特性和功能提供有价值的工具，也可能被开发用于潜在的临床应用。
