Guo Li-Ping, Huo Hong, Wang Xiao-Lei, Bu Zhi-Gao, Hua Rong-Hong
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, People's Republic of China .
Monoclon Antib Immunodiagn Immunother. 2014 Dec;33(6):438-43. doi: 10.1089/mab.2014.0047.
West Nile virus (WNV), which is an emerging pathogenic flavivirus with increasing distribution worldwide, is the cause of major human and animal health concerns. The pre-membrane (prM) protein of WNV is cleaved during maturation by the furin protease into the structural protein M and a pr-segment. In this study we generated and characterized a monoclonal antibody (MAb) against the WNV prM protein. Western blot analysis showed that the MAb reacted with WNV prM specifically. Immunohistochemistry assays demonstrated that the MAb recognized native prM protein in transfected BHK-21 cells. Preliminary studies were performed to identify the epitope recognized by the MAb using a set of synthesized overlapping peptides spanning the whole length of the prM protein. The MAb reported here may provide a valuable tool for the further exploration of the biological properties and functions of the prM protein and may also be developed for potential clinical applications.
西尼罗河病毒（WNV）是一种新兴的致病性黄病毒，在全球的分布日益广泛，是引起重大人类和动物健康问题的原因。WNV的前膜（prM）蛋白在成熟过程中被弗林蛋白酶切割成结构蛋白M和一个pr片段。在本研究中，我们制备并鉴定了一种针对WNV prM蛋白的单克隆抗体（MAb）。蛋白质印迹分析表明，该MAb与WNV prM特异性反应。免疫组织化学分析表明，该MAb可识别转染的BHK-21细胞中的天然prM蛋白。使用一组覆盖prM蛋白全长的合成重叠肽进行了初步研究，以鉴定该MAb识别的表位。本文报道的MAb可能为进一步探索prM蛋白的生物学特性和功能提供有价值的工具，也可能开发用于潜在的临床应用。
