抗除草剂精恶唑禾草灵单克隆抗体的制备。

Production of monoclonal antibody to herbicide fenoxaprop-ethyl.

作者信息

Cui Yongliang, Nan Tiegui, Tan Guiyu, Li Qing X, Wang Baomin, Liu Shangzhong

机构信息

College of Science, China Agricultural University, Beijing, People's Republic of China.

出版信息

Hybridoma (Larchmt). 2011 Oct;30(5):463-7. doi: 10.1089/hyb.2011.0054.

DOI:10.1089/hyb.2011.0054

PMID:22008074

Abstract

Fenoxaprop-ethyl is a selective aryloxyphenoxypropionate herbicide used widely to control annual and perennial grasses. A monoclonal antibody (MAb) against fenoxaprop-ethyl (FE), designated as 3E6B9C, was produced and had very low cross-reactivity with some of its structural analogs, such as clodinafop-propargyl, diclofop-methyl, lactofen, and quizalofop-p-ethyl. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed. The concentration of R-(+)-fenoxaprop-ethyl (R-FE) producing 50% of inhibition (IC(50)) and the working range of icELISA were 3.1 ng/mL and 0.6-29 ng/mL, respectively. This assay is also sensitive to R-fenoxaprop, S-(-)-fenoxaprop-ethyl, and metamifop with IC(50) of 3.4, 2.7, and 3.5 ng/mL, respectively. The recoveries of R-FE in soil samples with the icELISA were 86-102%.

摘要

精恶唑禾草灵是一种选择性芳氧苯氧丙酸类除草剂，广泛用于防除一年生和多年生禾本科杂草。制备了一种抗精恶唑禾草灵（FE）的单克隆抗体，命名为3E6B9C，它与其一些结构类似物，如炔草酯、禾草灵、乳氟禾草灵和精喹禾灵的交叉反应性非常低。建立了一种间接竞争酶联免疫吸附测定法（icELISA）。产生50%抑制率（IC(50)）的R-(+)-精恶唑禾草灵（R-FE）浓度和icELISA的工作范围分别为3.1 ng/mL和0.6 - 29 ng/mL。该测定法对R-精恶唑禾草灵、S-(-)-精恶唑禾草灵和苯氧喹啉也敏感，IC(50)分别为3.4、2.7和3.5 ng/mL。用icELISA测定土壤样品中R-FE的回收率为86% - 102%。