Sogut Ibrahim, Hatipoglu Ibrahim, Kanbak Gungor, Basalp Aynur
Genetic Engineering and Biotechnology Institute, Tubitak Marmara Research Center, Gebze-Kocaeli, Turkey.
Hybridoma (Larchmt). 2011 Oct;30(5):475-9. doi: 10.1089/hyb.2011.0052.
Despite effective vaccination programs in many countries, HBV infection is still a serious health problem throughout the world; more than 2 billion people have been infected with Hepatitis B virus (HBV). The serologic markers are crucial indicators in clinical diagnosis of HBV infection. The persistent presence of anti-HBc is associated with chronicity, and anti-HBe is an indicator for active viral replication. In the present study, two different hybridoma clones, 12E5 and 16F8, secreting anti-HBeAg and anti-HBc antibody were developed using hybridoma technology. BALB/c mice were immunized with HBe antigen (HBeAg), and monoclonal antibodies were generated from the spleen and lymph nodes of mice. Immunoglobulin types of antibodies were found to be IgG2a and IgG1, respectively. Monoclonal antibodies (MAbs) were produced in large scale, purified with affinity chromatography, and epitope analysis was performed. The results have shown that 12E5 and 16F8 monoclonal antibodies can be used for detection of HBcAg and HBeAg, indicating that they have the potential for use in clinical diagnosis.
尽管许多国家都有有效的疫苗接种计划，但乙肝病毒（HBV）感染在全球仍是一个严重的健康问题；超过20亿人已感染乙肝病毒。血清学标志物是乙肝病毒感染临床诊断的关键指标。抗-HBc的持续存在与慢性感染相关，而抗-HBe是病毒活跃复制的一个指标。在本研究中，利用杂交瘤技术制备了分泌抗-HBeAg和抗-HBc抗体的两种不同杂交瘤克隆12E5和16F8。用HBe抗原（HBeAg）免疫BALB/c小鼠，并从小鼠的脾脏和淋巴结产生单克隆抗体。发现抗体的免疫球蛋白类型分别为IgG2a和IgG1。大规模生产单克隆抗体（MAbs），用亲和层析法纯化，并进行表位分析。结果表明，12E5和16F8单克隆抗体可用于检测HBcAg和HBeAg，表明它们具有用于临床诊断的潜力。
