Ferjani Asma, Marzouk Manel, Idriss Nadia, Sammoud Sammoud, Hannachi Naila, Boukadida Janel
CHU Farhat Hached, Laboratoire de Microbiologie et D'immunologie UR02SP13, Sousse, Tunisie.
Ann Biol Clin (Paris). 2011 Sep-Oct;69(5):541-4. doi: 10.1684/abc.2011.0615.
We evaluated the performance and the cost of chromogenic medium Uriselect4 agar with regard to the standard medium for the detection and identification of urinary tract pathogens. A total of 503 clinical urine specimens containing leucocytes greater or equal to 104/mL were analysed prospectively, in parallel by two different persons on blood agar (GS) and Uriselect4 according to the manufacturers' instructions. Of the 503 urine specimens tested, 210 gave a positive culture on Uriselect4 versus 181 on GS. The majority of bacterial species grew on both media; enterobacteria grew on Uriselect4 better than GS. The identification of Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), KES group and Enterococcus faecalis (E. faecalis) did not require the use of galleries Api and has a gain of 24 h. Positive pure cultures on Uriselect4 corresponding to negative cultures of GS were noted in 17 ases. Conversely, in seven cases a positive pure culture on GS was noted while the corresponding Uriselect4 cultures were negative. The cost of identification on GS (including the cost of galleries Api), was about two times higher than Uriselect4. Uriselect4 medium isolates the most frequent urinary tract pathogens and identify them so almost immediately, with a lower cost.
我们评估了用于检测和鉴定尿路病原体的显色培养基Uriselect4琼脂相对于标准培养基的性能和成本。总共对503份白细胞计数大于或等于104/mL的临床尿液标本进行了前瞻性分析，由两名不同的人员按照制造商的说明，在血琼脂（GS）和Uriselect4上平行进行检测。在检测的503份尿液标本中，Uriselect4上有210份培养结果为阳性，而在GS上为181份。大多数细菌种类在两种培养基上均能生长；肠杆菌在Uriselect4上生长得比在GS上更好。大肠埃希菌（E. coli）、奇异变形杆菌（P. mirabilis）、KES组和粪肠球菌（E. faecalis）的鉴定无需使用Api鉴定条，且节省24小时。在17例病例中，Uriselect4上的阳性纯培养物对应于GS上的阴性培养物。相反，在7例病例中，GS上有阳性纯培养物，而相应的Uriselect4培养物为阴性。GS上的鉴定成本（包括Api鉴定条的成本）约为Uriselect4的两倍。Uriselect4培养基能分离出最常见的尿路病原体并几乎能立即对其进行鉴定，且成本较低。
