Navarro F, Fuentes I, Izquierdo C, Sánchez F, Prats G
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
Enferm Infecc Microbiol Clin. 1996 Apr;14(4):215-9.
The aim of the study was to evaluate the chromogenic agar plate CPS ID2 (bioMérieux) and determine its cost-benefit ratio.
A total of 2,193 urinary sediments were processed. The urine culture was carried out in CPS ID2 agar and in cystine-lactose electrolyte deficient (CLED) agar, when needed. Identification of the microorganisms was performed following standard microbiologic procedures through biochemical tests prepared in our laboratory. The identification, from CPS ID2 agar, by direct detection in medium of four metabolic activities: beta-glucuronidase, beta-glucosidase, deaminase, and indol production, was performed following to manufacturer's instructions.
A total of 289 urine cultures were positive, 18 were negative and 34 were contaminated samples. The identification, directly performed from the colonies detected in CPS ID2 agar, was correct in 96% of 166 Escherichia coli, in 92% of 24 Proteus mirabilis and in 97% of 38 enterococci. CPS ID2 agar exhibited 94% and 100% sensitivity and specificity, respectively in E. coli identification, 92% and 100% in P. mirabilis and 97% and 99% in Enterococcus. The use of this new media, CPS ID2, in our laboratory, implies a budgetary increment. However, if commercial galleries are used for routine identification, the cost will be reduced using this new media.
The CPS ID2 agar allows the isolation and direct identification of the most frequent urinary tract pathogens: E. coli, P. mirabilis and Enterococcus in primary isolation medium. Using this medium, bacteriologists will be able to save time and reagents when identifying the most common uropathogens. Furthermore, the use of this medium would reduce costs in some laboratories.
本研究旨在评估显色琼脂平板CPS ID2（生物梅里埃公司）并确定其成本效益比。
共处理了2193份尿沉渣。必要时，在CPS ID2琼脂和胱氨酸-乳糖电解质缺乏（CLED）琼脂中进行尿培养。通过我们实验室制备的生化试验，按照标准微生物学程序对微生物进行鉴定。根据制造商的说明，通过直接检测培养基中的四种代谢活性：β-葡萄糖醛酸酶、β-葡萄糖苷酶、脱氨酶和吲哚生成，从CPS ID2琼脂中进行鉴定。
共有289份尿培养阳性，18份阴性，34份为污染样本。从CPS ID2琼脂中检测到的菌落直接进行的鉴定，在166株大肠杆菌中有96%正确，在24株奇异变形杆菌中有92%正确，在38株肠球菌中有97%正确。CPS ID2琼脂在大肠杆菌鉴定中的敏感性和特异性分别为94%和100%，在奇异变形杆菌中为92%和100%，在肠球菌中为97%和99%。在我们实验室使用这种新培养基CPS ID2意味着预算增加。然而，如果使用商业鉴定板进行常规鉴定，使用这种新培养基成本将会降低。
CPS ID2琼脂可在初次分离培养基中分离并直接鉴定最常见的尿路病原体：大肠杆菌、奇异变形杆菌和肠球菌。使用这种培养基，细菌学家在鉴定最常见的尿路病原体时能够节省时间和试剂。此外，在一些实验室使用这种培养基将降低成本。
