Jha Rajeev Kumar, Xu Zi Rong, Shen Jian, Bai Shi Juan, Sun Jian Yu, Li Wei Fen
Genetic Engineering Laboratory, Feed Science Institute, Zhejiang University, 164, Qu Tao Bei Lu, Hangzhou 310029, China.
Immunol Lett. 2006 May 15;105(1):68-76. doi: 10.1016/j.imlet.2006.01.004. Epub 2006 Feb 13.
The effectiveness of oral, mock-, and immersion vaccination was investigated against white spot syndrome virus (WSSV) in crayfish. The most exposed WSSV envelope proteins VP19 and VP28 were used in different compositions and with different modes of applications. In experiment 1 crayfish were fed recombinant protein coated food pellets for 25 days, in experiment 2 the purified proteins were directly injected to them followed by one booster dose on 5th day and in experiment 3 the crayfish were left immersed in vaccines for 7 h. Experimental crayfish were challenged on 3rd and 21st days after last vaccination. The overall result showed that VP28 group has lowest cumulative mortality percentage accounting 39.6% at 3rd day and 39.83% at 21st day when injected and it was 43.2% and 49% when fed orally and 46.3% and 46.5% when immersed at 3rd and 21st days, respectively (p<0.05). In VP19 and VP28 (50:50) mixture, mock vaccination showed better performance (36.5%) over immersion (53%) and oral vaccination (53.2%) when challenged on 3rd day and mock vaccination (36.50%) followed by oral (51%) and immersion vaccination (56.5%) on 21st day as well. The VP19 recombinant mock vaccination group performed better (52.91% of 3rd day and 56.46% of 21st day) than oral (76% of 3rd day and 82% of 21st day) and immersion (83% of 3rd day and 86.3% of 21st day) vaccine groups. All the experimental groups except VP19 were significantly different (p<0.05) from the control groups. A positive cumulative effect was observed when VP28 was mixed with VP19 in equal proportion in all the experimental trials, which shows the effectiveness of VP19 as a vaccine component too. In the present trial on the basis of cumulative mortality percentage it is found that mock-vaccination group is more effective than the oral vaccination and immersion vaccination. It also suggests that specific memory exists in crayfish and the effects of VP28 are significant. The effect of VP19 along with VP28 has also shown significant effect against WSSV.
研究了口服、模拟和浸泡疫苗接种对小龙虾白斑综合征病毒（WSSV）的有效性。使用了最易暴露的WSSV包膜蛋白VP19和VP28，以不同的组合和不同的应用方式进行试验。在实验1中，小龙虾食用包被有重组蛋白的饲料颗粒25天；在实验2中，将纯化的蛋白直接注射到小龙虾体内，然后在第5天进行一次加强注射；在实验3中，将小龙虾浸泡在疫苗中7小时。在最后一次接种疫苗后的第3天和第21天对实验小龙虾进行攻毒。总体结果表明，VP28组的累积死亡率最低，注射时在第3天为39.6%，在第21天为39.83%；口服时在第3天和第21天分别为43.2%和49%；浸泡时在第3天和第21天分别为46.3%和46.5%（p<0.05）。在VP19和VP28（50:50）混合物中，在第3天攻毒时，模拟疫苗接种（36.5%）的表现优于浸泡接种（53%）和口服接种（53.2%）；在第21天攻毒时，模拟疫苗接种（36.50%）之后依次是口服接种（51%）和浸泡接种（56.5%）。VP19重组模拟疫苗接种组（第3天为52.91%，第21天为56.46%）的表现优于口服疫苗接种组（第3天为76%，第21天为82%）和浸泡疫苗接种组（第3天为83%，第21天为86.3%）。除VP19组外，所有实验组与对照组均有显著差异（p<0.05）。在所有实验中，当VP28与VP19等比例混合时观察到了积极的累积效应，这也表明VP19作为疫苗成分的有效性。在本次试验中，基于累积死亡率发现模拟疫苗接种组比口服疫苗接种组和浸泡疫苗接种组更有效。这也表明小龙虾存在特异性记忆，并且VP28的效果显著。VP19与VP28联合使用对WSSV也显示出显著效果。
