开放式自动化核酸提取仪在加蓬（中非）进行高通量血浆 HIV-1 RNA 定量检测的适用性。
Suitability of an open automated nucleic acid extractor for high-throughput plasma HIV-1 RNA quantitation in Gabon (Central Africa).
机构信息
UMI 233, Institut de Recherche pour le Développement (IRD), University of Montpellier, Montpellier, France.
出版信息
J Virol Methods. 2012 Jan;179(1):269-71. doi: 10.1016/j.jviromet.2011.10.001. Epub 2011 Oct 10.
Nucleic acid extraction using the open automated EZ1 (Qiagen) instrument, in combination with the Generic HIV Viral Load assay, gave highly concordant HIV-1 RNA viral load results among 181 Gabonese subjects infected with HIV-1, compared to those obtained when performing a manual extraction. Since people living with HIV-1 are being treated with antiretrovirals in Gabon, this automated extraction technique represents an excellent technical method for high-throughput monitoring of HIV-1 RNA viral load.
采用开放式自动化 EZ1（Qiagen）仪器进行核酸提取，结合通用 HIV 病毒载量检测试剂盒，与手动提取相比，在 181 名感染 HIV-1 的加蓬受试者中得到了高度一致的 HIV-1 RNA 病毒载量结果。由于加蓬的 HIV-1 感染者正在接受抗逆转录病毒治疗，这种自动化提取技术代表了一种用于高通量监测 HIV-1 RNA 病毒载量的出色技术方法。
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