Department of Pediatric Dentistry and Orthodontics, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.
Orthod Craniofac Res. 2011 Nov;14(4):234-42. doi: 10.1111/j.1601-6343.2011.01528.x.
The objective of this study is to examine the effect of cyclosporine-A (CsA) on the rate of orthodontic tooth movement in rats.
This is a randomized controlled trial with a split-mouth design in Sprague-Dawley rats.
Eighteen rats, divided at random in two groups, were fed with 8 mg/kg CsA (experiment) or mineral oil (control) daily after initial healing of bilateral maxillary second molar removal. All rats received orthodontic coil springs (10 cN) secured to the maxillary incisors and first molars at the rights side, while no springs were placed at the left. Distances between first and third molars were measured on days 0, 3, 6, and 12. After sacrificing on day 12, the alveolar ridges of the maxillae were sectioned and blood samples were collected for serum tartrate-resistant acid phosphatase (TRAP)-5b level detection and for histology, respectively.
Significantly larger changes in intermolar distances were found after orthodontic force application in the CsA group at days 3 and 12 when compared with the control group. The inter-radicular dental alveolus of CSA-fed rats was osteopenic. Significantly increased TRAP-5b serum level was noted in the CsA group when compared with the control group.
We suggest that CsA enhanced the rate of orthodontic tooth movement. The osteopenia and the increased osteoclastic activity could be the underlying factors.
本研究旨在探讨环孢素 A（CsA）对大鼠正畸牙移动速度的影响。
这是一项随机对照试验，采用 Sprague-Dawley 大鼠的分侧设计。
18 只大鼠随机分为两组，在双侧上颌第二磨牙拔除后初始愈合期每天分别给予 8mg/kgCsA（实验组）或矿物油（对照组）。所有大鼠右侧上颌切牙和第一磨牙上均固定正畸螺旋弹簧（10cN），左侧不放置弹簧。在第 0、3、6 和 12 天测量第一和第三磨牙之间的距离。第 12 天处死大鼠后，分别对上颌牙槽嵴进行切片，并采集血样检测血清抗酒石酸酸性磷酸酶 5b（TRAP-5b）水平和进行组织学检查。
与对照组相比，CsA 组在正畸力应用后的第 3 和 12 天，牙间距离的变化明显更大。CSA 喂养大鼠的根间牙槽骨呈骨质疏松症。与对照组相比，CsA 组血清 TRAP-5b 水平显著升高。
我们认为 CsA 可加速正畸牙移动。骨吸收和破骨细胞活性增加可能是其潜在的作用机制。
