School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Fitoterapia. 2012 Jan;83(1):117-24. doi: 10.1016/j.fitote.2011.10.001. Epub 2011 Oct 10.
A sensitive liquid chromatographic-mass spectrometric technique coupled with liquid-liquid extraction method was developed and validated for simultaneous determination of dehydro-tumulosic acid, tumulosic acid and polyporic acid C in rat plasma. The analytes were separated on a Kromasil C(18) column with a total running time of 12.5 min. Author had compared the pharmacokinetics of dehydro-tumulosic acid, tumulosic acid and polyporic acid C after oral administration of the extract of Poria and its formulated herbal preparation (GuiZhi-FuLing capsule). The improved pharmacokinetic profiles of the three compounds were found in the GuiZhi-FuLing capsule, indicating the more effective absorption and the slower elimination, compared with the Poria extract. Furthermore, this study revealed that as far as the Poria extract was concerned, it is very valuable to be used as a clinical instruction of GF capsule.
建立并验证了一种灵敏的液相色谱-质谱联用技术，结合液-液萃取法，用于同时测定大鼠血浆中的去氢土莫酸、土莫酸和多孔菌酸 C。采用 Kromasil C（18）柱，总运行时间为 12.5 分钟，实现了分析物的分离。作者比较了口服茯苓及其配方制剂（桂芝茯苓胶囊）提取物后去氢土莫酸、土莫酸和多孔菌酸 C 的药代动力学。与茯苓提取物相比，桂芝茯苓胶囊中三种化合物的药代动力学特征得到了改善，表明其吸收更有效，消除更缓慢。此外，本研究表明，就茯苓提取物而言，它非常有价值，可以作为 GF 胶囊的临床指导。
