College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
J Pharm Biomed Anal. 2013 Mar 25;76:215-8. doi: 10.1016/j.jpba.2012.12.004. Epub 2012 Dec 22.
A specific and sensitive high performance liquid chromatography-mass spectrometric (HPLC-MS) method has been developed and validated for simultaneous determination of three anthraquinones of rhein, aloe-emodin and emodin in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. The analytes were separated on a Kromaisl(®) C(18) column within a total running time of 12min with a mobile phase of methanol:ammonium acetate (3mM) (75:25, v/v). The calibration curves for all the anthraquinones showed good linearity in the measured range with correlation coefficient (r) higher than 0.9978. The precision, accuracy, recovery and stability were deemed acceptable. The method was successfully applied to the comparative pharmacokinetics study of the anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang.
建立并验证了一种专属性强、灵敏度高的高效液相色谱-质谱（HPLC-MS）法，用于测定大鼠灌胃给予含大黄的复方制剂后血浆中三种蒽醌类成分（大黄酸、芦荟大黄素和大黄素）的浓度。采用 Kromasil(®) C(18)色谱柱，以甲醇-3mM 乙酸铵（75:25，v/v）为流动相，在 12min 内完成分析。所有蒽醌类成分在测定范围内线性关系良好，相关系数（r）均大于 0.9978。该方法的精密度、准确度、回收率和稳定性均良好。该方法已成功用于大鼠灌胃给予含大黄的复方制剂后血浆中蒽醌类成分的比较药代动力学研究。
