Research Laboratories for Brewing, Asahi Breweries, Ltd., 1-21, Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan.
Biotechnol Lett. 2012 Feb;34(2):347-51. doi: 10.1007/s10529-011-0777-3. Epub 2011 Oct 19.
α-Amylase activities of Aspergillus oryzae grown on dextrin or indigestible dextrin were 7·8 and 27·7 U ml(-1), respectively. Glucoamylase activities of the cultures grown on dextrin or indigestible dextrin were 5·4 and 301 mU ml(-1), respectively. The specific glucoamylase production rate in indigestible dextrin batch culture reached 1·35 U g DW(-1) h(-1). In contrast, biomass concentration of A. oryzae in indigestible dextrin culture was 35% of that in dextrin culture. Thus, the culture method using indigestible dextrin has the potential to improve amylolytic enzyme production and fungal fermentation broth rheology.
米曲霉在糊精或难消化糊精上生长时的α-淀粉酶活性分别为 7.8 和 27.7 U/ml。在糊精或难消化糊精上生长的培养物的糖化酶活性分别为 5.4 和 301 mU/ml。在难消化糊精分批培养中，特异性糖化酶的产率达到 1.35 U/g DW/h。相比之下，米曲霉在难消化糊精培养物中的生物量浓度为在糊精培养物中的 35%。因此，使用难消化糊精的培养方法具有提高淀粉分解酶生产和真菌发酵液流变性的潜力。
