Departamento Sanidad Animal y Medicina Preventiva, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.
Poult Sci. 2011 Nov;90(11):2638-41. doi: 10.3382/ps.2011-01621.
Enteropathogenic Escherichia coli is a foodborne pathogen that produces potentially fatal infant diarrhea, noticeably in developing countries. The aim of this study was to detect EPEC contamination by PCR at different stages of the chicken slaughtering process. We collected swabs from chicken cloacae and washed carcasses (external and visceral cavity) during the slaughtering process in 3 sampling occasions. Unwashed eviscerated carcasses were also sampled (at the visceral cavity) in the second and third sampling occasions. Enteropathogenic Escherichia coli was detected in 6 to 28% of cloacal samples, 39 and 56% of unwashed eviscerated carcasses, and 4 to 58% of washed carcasses. None of the samples were positive for bfpA, suggesting contamination with atypical EPEC. The detection of EPEC at different stages of the chicken slaughtering process showed that the proportion of contaminated samples remained or even increased during processing. In addition, the high proportion of contaminated carcasses during chicken processing represents a risk for the consumers and a challenge to improve procedures for those working in the sanitary control service.
肠致病性大肠杆菌是一种食源性病原体，可导致婴儿致命性腹泻，在发展中国家尤为明显。本研究旨在通过 PCR 检测鸡肉屠宰过程不同阶段的 EPEC 污染情况。我们在 3 个采样时间点从鸡泄殖腔采集拭子，并在屠宰过程中清洗屠体（外部和内脏腔）。在第二和第三个采样时间点，还对未清洗的去内脏屠体（内脏腔）进行了采样。在 6 至 28%的泄殖腔样本、39%和 56%的未清洗去内脏屠体以及 4%至 58%的清洗屠体中检测到肠致病性大肠杆菌。没有样本对 bfpA 呈阳性，提示存在非典型 EPEC 污染。在鸡肉屠宰过程的不同阶段检测到 EPEC 表明，在加工过程中，污染样本的比例保持或甚至增加。此外，鸡肉加工过程中大量受污染的屠体对消费者构成风险，对从事卫生控制服务的人员提出了改进程序的挑战。
