Adorján András, Makrai László, Mag Tünde, Jánosi Szilárd, Könyves László, Tóth István
Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
National Public Health Center, Budapest, Hungary.
Front Vet Sci. 2020 Aug 13;7:511. doi: 10.3389/fvets.2020.00511. eCollection 2020.
(EC) strains belong to several pathotypes capable of infecting both humans and animals. Some of them have zoonotic potential and can sporadically cause epidemic outbreaks. Our aim was to screen for the distribution of these pathotypes in broilers and their related products. Therefore, strains were isolated ( = 118) from poultry intestine ( = 57), carcass ( = 57), and wastewater ( = 4) samples from one slaughterhouse with own reared poultry source and the National Reference Laboratory (NRL) poultry collection ( = 170) from the year 2017 was also studied. All 288 strains were screened by PCR for pathotype-specific genes , and for further EPEC-specific virulence genes (). Altogether 35 atypical enteropathogenic (aEPEC) strains from the slaughterhouse and 48 aEPEC strains from the NRL collection were found. Regarding the phylogenetic groups of aEPEC, all four main groups were represented but there was a shift toward the B2 group (25%) as compared with the non-EPEC isolates (3%). The aEPEC isolates belonged to serogroups O14, O108, and O45. Multidrug resistance (MDR) was abundant in aEPEC strains (80 out of 83 aEPEC) with a diverse resistance pattern ( = 56). Our results of this study indicate that the high frequency of aEPEC in broilers and on their carcass surface, with frequent MDR to several antibiotic groups, raises the possibility that these strains pose a zoonotic risk to humans.
肠出血性大肠杆菌（EC）菌株属于几种能够感染人类和动物的致病型。其中一些具有人畜共患病潜力，可偶尔引发疫情爆发。我们的目的是筛查这些致病型在肉鸡及其相关产品中的分布情况。因此，从一家有自有养殖家禽来源的屠宰场的家禽肠道（n = 57）、胴体（n = 57）和废水（n = 4）样本中分离出菌株（n = 118），并对2017年国家参考实验室（NRL）的家禽菌株 collection（n = 170）进行了研究。通过PCR对所有288株大肠杆菌菌株进行致病型特异性基因的筛查，并进一步检测肠致病性大肠杆菌（EPEC）特异性毒力基因（）。共从屠宰场分离出35株非典型肠致病性大肠杆菌（aEPEC）菌株，从NRL collection中分离出48株aEPEC菌株。关于aEPEC的系统发育组，所有四个主要组均有代表，但与非EPEC分离株（3%）相比，向B2组（25%）有所偏移。aEPEC分离株属于O14、O108和O45血清型。aEPEC菌株中多重耐药（MDR）情况普遍（83株aEPEC中有80株），耐药模式多样（n = 56）。我们的研究结果表明，肉鸡及其胴体表面aEPEC的高频率存在，且对多种抗生素组频繁出现MDR，增加了这些菌株对人类构成人畜共患病风险的可能性。
