Xie Ting-Ting, Qiu Feng, Yang Mei-Hua, Qi Ai-Di
Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China.
Yao Xue Xue Bao. 2011 Jul;46(7):822-7.
A rapid and sensitive analytical method was developed for the simultaneous determination of fumonisins B1 and B2 in traditional Chinese medicines by HPLC-MS/MS. The detection limits for fumonisins B1 and B2 were 0.25 ng x mL(-1) corresponding to 2 microg x kg(-1) in samples. Recoveries from different samples spiked with fumonisins B1 and B2 at levels ranging from 0.2 to 3 mg x kg(-1) were 84.0%-96.1% and 86.3%-99.3%, respectively. Among the total of 34 samples purchased from local markets, ten samples of which were visibly moldy samples due to inappropriate storage, and 24 were normal samples. The results showed that 6 of the visibly moldy samples and 5 of the normal samples were contaminated with total fumonisins at levels ranging 82.4-2349 microg x kg(-1) and 102-729 microg x kg(-1), respectively.
建立了一种快速灵敏的HPLC-MS/MS分析方法，用于同时测定中药中的伏马毒素B1和B2。伏马毒素B1和B2的检测限为0.25 ng·mL(-1)，相当于样品中2 μg·kg(-1)。在添加了浓度范围为0.2至3 mg·kg(-1)的伏马毒素B1和B2的不同样品中的回收率分别为84.0%-96.1%和86.3%-99.3%。从当地市场购买的34个样品中，有10个样品因储存不当而明显发霉，24个为正常样品。结果表明，6个明显发霉的样品和5个正常样品被总伏马毒素污染，污染水平分别为82.4-2349 μg·kg(-1)和102-729 μg·kg(-1)。
