Zheng Run-Sheng, Xu Hui, Peng Yuan-Xia, Wang Wen-Li, Zhan Ruo-Ting, Chen Wei-Wen
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):273-7.
As the dilution procedure was applied, a simple, rapid and cost-effective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of aflatoxin B1, B2, G1, and G2 was successfully by performed in a total 83 samples of 10 traditional Chinese medicines (TCMs), which were collected from 5 different hospital pharmacies and 5 different medical stores in Guangzhou city. Matrix effects of these 10 TCMs were ranged from 80.23% to 115.5% in low, intermediate and high concentration levels, indicating that the negative effect was overcome in this study. Meanwhile, the analysis method was proved to be stable and reliable during the whole analysis using Semen Armeniacae Amarum spiked 3 concentration levels of standard solution as quality control samples and the RSD < 6.6% was obtained. The contamination levels of 83 investigated samples were 13.89% and 17.02% in hospital pharmacies and medical stores, respectively. The result was presented to provide relevant reference and supplement to those researchers in TCMs analysis and screening.
采用稀释程序后，成功建立了一种简单、快速且经济高效的高效液相色谱 - 串联质谱法（LC-MS/MS），用于测定从广州市5家不同医院药房和5家不同药店采集的10种中药共83个样品中的黄曲霉毒素B1、B2、G1和G2。这10种中药在低、中、高浓度水平下的基质效应范围为80.23%至115.5%，表明本研究克服了负面影响。同时，以苦杏仁加标3个浓度水平的标准溶液作为质量控制样品，在整个分析过程中证明该分析方法稳定可靠，相对标准偏差（RSD）<6.6%。在调查的83个样品中，医院药房和药店的污染水平分别为13.89%和17.02%。该结果可为中药分析和筛选领域的研究人员提供相关参考和补充。
