Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Int J Immunogenet. 2012 Feb;39(1):15-9. doi: 10.1111/j.1744-313X.2011.01049.x. Epub 2011 Oct 20.
As certain cytokines may play a role in unexplained recurrent spontaneous abortion (RSA) and also some cytokine gene polymorphisms may affect the level of cytokine production, the aim of this study was to investigate the relationship between Chinese RSA and polymorphisms of the genes coding for interleukin (IL)-1β (-31C/T, -511C/T, +3954C/T) and IL-6 (-634C/G). Women (n = 162) with at least three consecutive spontaneous abortions and 156 ethnically matched healthy women with at least one successful pregnancy were included. Genotypes were determined using restriction fragment length polymorphism analysis of polymerase chain reaction products. No significant differences were found in the IL-1β-31T, -511T and +3954T distributions between the RSA group and the control group. On the other hand, the frequencies of the IL-6-634GG genotype and -634G allele were significantly decreased in the RSA group versus the control group (genotype: P = 0.0003; allele: P = 0.002), suggesting the IL-6-634C/G polymorphism might be a possible genetic protective factor for RSA.
由于某些细胞因子可能在不明原因的复发性自然流产 (RSA) 中发挥作用，并且某些细胞因子基因多态性可能影响细胞因子的产生水平，因此本研究旨在探讨中国 RSA 与白细胞介素 (IL)-1β (-31C/T、-511C/T、+3954C/T) 和 IL-6 (-634C/G) 编码基因多态性之间的关系。纳入了至少连续发生 3 次自然流产的 162 名女性和至少有 1 次成功妊娠的 156 名具有相同种族的健康女性。使用聚合酶链反应产物的限制性片段长度多态性分析确定基因型。在 RSA 组和对照组之间，IL-1β-31T、-511T 和 +3954T 分布无显著差异。另一方面，与对照组相比，IL-6-634GG 基因型和 -634G 等位基因的频率在 RSA 组中显著降低（基因型：P = 0.0003；等位基因：P = 0.002），表明 IL-6-634C/G 多态性可能是 RSA 的一种潜在遗传保护因素。
