Department of Biopathological Science and Hygiene of Animal Production and Foods, University of Perugia, Perugia, Italy.
Foodborne Pathog Dis. 2012 Jan;9(1):75-8. doi: 10.1089/fpd.2011.0930. Epub 2011 Oct 19.
Serum and meat juice analyses for the detection of Toxoplasma gondii antibodies by an immunofluorescence antibody assay were compared in 100 seropositive and 100 seronegative slaughtered heavy swine. Meat juice was obtained from diaphragm and gracilis muscles of the serologically tested animals. Seventy-two diaphragmatic meat juice samples (36%, 95% interval confidence [IC] 29.4%-43.1%) and 63 gracilis meat juice samples (31.5%, 95% IC 25.1%-38.4%) tested positive for T. gondii antibodies. The average concordance between serum and meat juice derived from both muscles was "substantial" (K=0.6-0.8). The K-value was 1 when considering serum samples showing a titer >1/16, whereas it decreased to 0.62 and to 0.49 when considering serum samples with a 1/16 titer in meat juice from diaphragm and gracilis muscle, respectively.
采用免疫荧光抗体检测法对 100 份血清阳性和 100 份血清阴性屠宰肥猪的血清和肉汁进行了弓形虫抗体检测分析。肉汁取自经血清学检测的动物的横膈膜和股薄肌。72 份横膈膜肉汁样本（36%，95%置信区间[CI] 29.4%-43.1%）和 63 份股薄肌肉汁样本（31.5%，95% CI 25.1%-38.4%）的弓形虫抗体检测呈阳性。来自两种肌肉的血清和肉汁之间的平均一致性为“高度”（K=0.6-0.8）。当考虑血清样本的滴度>1/16 时，K 值为 1，而当考虑血清样本在横膈膜和股薄肌的肉汁中的 1/16 滴度时，K 值分别降至 0.62 和 0.49。
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