Carella Emanuele, Caruso Claudio, Moreno Ana, Di Blasio Alessia, Oberto Francesca, Vitale Nicoletta, Masoero Loretta
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d' Aosta, Via Bologna 148, 10154 Torino, Italy.
Azienda Sanitaria Locale CN1 (ASL-CN1), Via Pier Carlo Boggio 12, 12100 Cuneo, Italy.
Microorganisms. 2023 Sep 27;11(10):2418. doi: 10.3390/microorganisms11102418.
Aujeszky Disease Virus (ADV) is a double-stranded DNA virus with a lipoprotein envelope. The natural hosts of the infection are Suidae, but the virus can infect many other mammals. The gold-standard method identified by the WOAH for the diagnosis of Aujeszky disease is the ELISA method. The objective of this study was to compare the performance of meat juice and oral fluid matrices using a commercial ELISA kit designed for serum. A total of 80 blood and oral fluid samples were collected from four pig farms selected for this study. Diaphragm muscle samples of about 100 g and blood samples were collected from 213 animals at the abattoir. These biological matrices were collected from the same animals and tested using a competitive ELISA kit to detect antibodies against ADV. The relative accuracy of the meat juice compared to that of the serum was 96.7% (95% CI: 93.3-98.7%), with 206 correct results out of 213. The relative accuracy of the oral fluid compared to that of the serum was 61.3% (95% CI: 49.7-71.9%), with 58 correct results out of 80. Meat juice has a better combination of sensitivity and specificity than oral fluid. The usage of meat juice in routine diagnostic examinations could be achieved after further investigations to optimize the procedure.
伪狂犬病病毒（ADV）是一种具有脂蛋白包膜的双链DNA病毒。该感染的天然宿主是猪科动物，但该病毒可感染许多其他哺乳动物。世界动物卫生组织确定的伪狂犬病诊断的金标准方法是酶联免疫吸附测定（ELISA）法。本研究的目的是使用一种专为血清设计的商业ELISA试剂盒，比较肉汁和口腔液体样本的检测性能。从为本研究选定的四个养猪场共采集了80份血液和口腔液体样本。在屠宰场从213只动物身上采集了约100克的膈肌肌肉样本和血液样本。这些生物样本取自同一批动物，并使用竞争ELISA试剂盒检测抗ADV抗体。与血清相比，肉汁的相对准确率为96.7%（95%置信区间：93.3 - 98.7%），213份样本中有206份结果正确。与血清相比，口腔液体的相对准确率为61.3%（95%置信区间：49.7 - 71.9%），80份样本中有58份结果正确。肉汁在敏感性和特异性方面的综合表现优于口腔液体。经过进一步研究以优化检测程序后，可在常规诊断检查中使用肉汁样本。
