用于确认疑似γ-羟基丁酸中毒的干血斑冲孔样本：优化气相色谱-质谱联用程序的验证
Dried blood spot punches for confirmation of suspected γ-hydroxybutyric acid intoxications: validation of an optimized GC-MS procedure.
作者信息
Ingels Ann-Sofie, De Paepe Peter, Anseeuw Kurt, Van Sassenbroeck Diederik, Neels Hugo, Lambert Willy, Stove Christophe
机构信息
Laboratory of Toxicology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
出版信息
Bioanalysis. 2011 Oct;3(20):2271-81. doi: 10.4155/bio.11.204.
BACKGROUND
γ-hydroxybutyric acid (GHB), notorious as a club- and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by 'on-spot' derivatization and analysis by GC-MS.
RESULTS
A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 20-35 µl spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2-100 µg/ml.
CONCLUSION
A sensitive GC-MS method for GHB analysis in DBS was successfully optimized and validated. The successful analysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB analysis in toxicological cases.
背景
γ-羟基丁酸（GHB）作为一种在夜店和约会时使用的强奸药物而声名狼藉，通过冲出圆盘状干血斑（DBS），随后进行“现场”衍生化并采用气相色谱-质谱联用（GC-MS）分析对其进行定量。
结果
证明了在干血斑中分布均匀，并且当分析从20 - 35微升血斑冲出的圆盘时，无论血样的血细胞比容如何，均可获得准确结果。根据美国食品药品监督管理局（US FDA）和欧洲药品管理局的指南进行了验证，校准范围为2 - 100微克/毫升。
结论
成功优化并验证了一种用于干血斑中GHB分析的灵敏GC-MS方法。对从GHB滥用者处采集的干血斑进行的成功分析表明，干血斑采样技术在毒理学案例中对GHB分析具有常规适用性。
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