Department of Anatomy and Cell Biology, School of Medicine, Zhejiang University, Zhejiang 310058, PR China.
Mol Med Rep. 2012 Feb;5(2):405-9. doi: 10.3892/mmr.2011.639. Epub 2011 Oct 19.
Renal preservation is a universal problem since ischemia/reperfusion (I/R) injury remains an unresolved issue during the procedure of renal transplantation. Tanshinone IIA, one of the effective components of the traditional Chinese medicine Danshen, was reported to exhibit a variety of biochemical activities, including protection against I/R injury. Therefore, identifying the specific molecular pathway mediating tanshinone IIA protection of renal preservation would be of great value to the patients concerned. In this study, rats were divided into two groups and the kidneys were isolated and preserved in two solutions separately, one with Celsior solution and the other with tanshinone IIA additionally added to the Celsior solution. The superoxide dismutase (SOD) activity and the quantity of malonaldehyde (MDA) were measured, the expression of CHOP and caspase-12 were assessed by immunohistochemistry staining, and real-time quantitative reverse transcription-polymerase chain reaction analysis was performed after 0, 24 and 48 h of preservation. A significant increase in the activities of SOD and a decrease in the quantity of MDA were observed in the kidneys preserved with tanshinone IIA at 24 and 48 h (P<0.01). The expression of CHOP and caspase-12 was lower in the kidneys preserved with tanshinone IIA at 24 and 48 h than that in the kidneys preserved with Celsior solution alone (P<0.05). The results suggest that the supplementation of tanshinone IIA in standard Celsior solution may significantly improve long-term kidney preservation. Attenuating oxidative stress injury and decreasing endoplasmic reticulum (ER) stress‑mediated apoptosis may play a role in the protection of kidney hypothermic preservation.
肾脏保存是一个普遍存在的问题，因为缺血/再灌注（I/R）损伤仍然是肾移植过程中尚未解决的问题。丹参酮 IIA 是中药丹参的有效成分之一，据报道具有多种生化活性，包括对 I/R 损伤的保护作用。因此，确定介导丹参酮 IIA 保护肾脏保存的特定分子途径对于相关患者具有重要意义。在这项研究中，大鼠分为两组，肾脏分别在两种溶液中分离和保存，一种是 Celsior 溶液，另一种是 Celsior 溶液中额外添加丹参酮 IIA。测量超氧化物歧化酶（SOD）活性和丙二醛（MDA）的含量，通过免疫组织化学染色评估 CHOP 和 caspase-12 的表达，以及在保存 0、24 和 48 h 后进行实时定量逆转录-聚合酶链反应分析。在 24 和 48 h 时，用丹参酮 IIA 保存的肾脏中 SOD 活性显著增加，MDA 含量减少（P<0.01）。在 24 和 48 h 时，用丹参酮 IIA 保存的肾脏中 CHOP 和 caspase-12 的表达低于单用 Celsior 溶液保存的肾脏（P<0.05）。结果表明，在标准 Celsior 溶液中补充丹参酮 IIA可能显著改善长期肾脏保存。减轻氧化应激损伤和减少内质网（ER）应激介导的细胞凋亡可能在肾脏低温保存的保护中发挥作用。
