Division of Nutritional Sciences, School of Biosciences, Sutton Bonington Campus, The University of Nottingham, Leicestershire LE12 5RD, UK.
J Muscle Res Cell Motil. 2012 Mar;32(6):383-90. doi: 10.1007/s10974-011-9267-4. Epub 2011 Oct 20.
The regulation of muscle fibre transitions has mainly been studied in vivo using conventional histological or immunohistochemical techniques. In order to investigate the molecular regulation of myosin heavy chain (MyHC) isoform expression in cell culture studies, we first characterised the normal transitions in endogenous expression of the MyHC isoforms and the myogenic regulatory factors during differentiation of C2C12 muscle cells. Interestingly, across the time course of differentiation, MyHC mRNA isoforms were expressed in a distinct temporal pattern as two distinct cohorts, one including MyHC I, embryonic and neonatal, the other including MyHC IIa, IIx and IIb. The pattern of expression suggests a transition in MyHC isoforms, from one cohort to another, occurs during muscle cell differentiation and that these transitions occur independent of nerve innervation. To our knowledge, this is the most comprehensive analysis of in vitro MyHC mRNA isoform transitions and provides important information for studying the regulation of transitions in MyHC isoforms in cell culture systems.
肌肉纤维转变的调控主要通过传统的组织学或免疫组织化学技术在体内进行研究。为了在细胞培养研究中研究肌球蛋白重链（MyHC）同工型表达的分子调控，我们首先描述了 C2C12 肌肉细胞分化过程中内源性 MyHC 同工型和肌生成调节因子表达的正常转变。有趣的是，在分化的整个过程中，MyHC mRNA 同工型以两种不同的簇表现出明显的时间模式表达，一个包括 MyHC I、胚胎和新生，另一个包括 MyHC IIa、IIx 和 IIb。这种表达模式表明，在肌肉细胞分化过程中，MyHC 同工型从一个簇转变为另一个簇，并且这些转变独立于神经支配。据我们所知，这是体外 MyHC mRNA 同工型转变的最全面分析，并为研究细胞培养系统中 MyHC 同工型转变的调控提供了重要信息。
