Health Science and Technology Interdepartmental Center for Industrial Research, University of Bologna, Ozzano Emilia, Bologna, Italy.
Dev Dyn. 2011 Nov;240(11):2482-94. doi: 10.1002/dvdy.22761.
The establishment of rat embryonic stem cells constitutes a precious tool since rat has been extensively used in biomedical research, in particular for the generation of human neurodisease animal models. Up to now only a few studies have described the isolation of rat embryonic stem-like cells. One out of 9 isolated rat embryonic stem-like cell lines (B1-RESC) obtained from a 4.5-day post-coitum blastocyst were extensively characterized and kept in culture for up to 80 passages on feeders with LIF. The stable growth of these cells and the expression of pluripotent markers were confirmed up to a high number of passages in culture, also in the absence of feeders and LIF. B1-RESC expresses the three germ layers markers both in vitro, within differentiating embryoid bodies, and in vivo through teratoma formation. Collectively, the B1-RESC line with a stable near-diploid karyotype can be used as a highly sensitive tool for testing anti-proliferative molecules.
大鼠胚胎干细胞的建立是一个宝贵的工具，因为大鼠在生物医学研究中被广泛应用，特别是用于生成人类神经疾病动物模型。迄今为止，只有少数研究描述了大鼠胚胎样干细胞的分离。从 4.5 天的囊胚中分离得到的 9 株大鼠胚胎样干细胞系（B1-RESC）之一，经过广泛的特征描述，并在饲养层上使用 LIF 培养，最多可达 80 代。这些细胞的稳定生长和多能性标记物的表达在培养过程中，甚至在没有饲养层和 LIF 的情况下，也得到了确认，达到了很高的代次。B1-RESC 在体外、分化的胚胎体中以及体内通过畸胎瘤形成表达三个胚层标记物。总的来说，具有稳定的近二倍体核型的 B1-RESC 系可作为一种高度敏感的工具，用于测试抗增殖分子。
