Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Mol Cell Biochem. 2012 Feb;361(1-2):281-8. doi: 10.1007/s11010-011-1113-x. Epub 2011 Oct 20.
Methylphenidate (MPH), a psychostimulant that affects both dopaminergic and noradrenergic systems, is one of the most frequently prescribed treatments for attention-deficit hyperactivity disorder. The present study investigated the effects of chronic administration of MPH on some parameters of oxidative stress, as well as on butyrylcholinesterase (BuChE) activity in blood of young rats. Rats received intraperitoneal injections of MPH (2.0 mg/kg) once a day, from the 15th to the 45th day of age or an equivalent volume of 0.9% saline solution (controls). Two hours after the last injection, animals were euthanized, and blood was collected. Results demonstrated that MPH did not alter the dichlorofluorescein formed, decreased both thiobarbituric acid reactive substances and total non-enzymatic radical-trapping antioxidant, and increased superoxide dismutase and catalase activities, suggesting that this psychostimulant may alter antioxidant defenses. BuChE activity was increased in blood of juvenile rats subjected to chronic MPH administration. These findings suggest that MPH may promote peripheral oxidative adaptations and cholinergic changes.
哌醋甲酯（MPH）是一种影响多巴胺能和去甲肾上腺素能系统的精神兴奋剂，是治疗注意力缺陷多动障碍最常用的药物之一。本研究探讨了慢性给予 MPH 对年轻大鼠血液中一些氧化应激参数以及丁酰胆碱酯酶（BuChE）活性的影响。大鼠从第 15 天到第 45 天每天接受腹膜内注射 MPH（2.0mg/kg）或等体积的 0.9%生理盐水（对照组）。最后一次注射后 2 小时，处死动物并采集血液。结果表明，MPH 不会改变二氯荧光素的形成，减少丙二醛反应物质和总非酶自由基捕获抗氧化剂，增加超氧化物歧化酶和过氧化氢酶的活性，表明这种精神兴奋剂可能改变抗氧化防御。慢性给予 MPH 可增加幼年大鼠血液中的 BuChE 活性。这些发现表明，MPH 可能促进外周氧化适应和胆碱能变化。
