Clean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, Japan.
J Mass Spectrom. 2011 Oct;46(10):967-75. doi: 10.1002/jms.1977.
Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents.
去污剂在从生物细胞或组织中提取蛋白质的不同提取方案中是必要的。提取后，为了更好地进行电喷雾电离质谱（ESI-MS）分析，需要去除去污剂。去除去污剂既费力又费时，而且还不可避免地会损失样品。我们实验室开发的探针电喷雾（PESI）已被发现对样品溶液中盐和缓冲液的存在具有耐受性。在本报告中，研究了 PESI 是否适用于含有高浓度去污剂的样品溶液。结果发现，与传统 ESI 和纳喷 ESI 相比，PESI 对十二烷基硫酸钠、十六烷基三甲基溴化铵、Triton X100 和 3-[(3-胆酰胺丙基)二甲氨基]-1-丙磺酸钠的存在具有很高的耐受性。因此，PESI 可以成为一种潜在的分析工具，用于直接分析含有高浓度去污剂的蛋白质提取物和消化物。
