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探针电喷雾电离（PESI）及其改进版本：浸入式PESI（dPESI）、鞘流PESI（sfPESI）和可调鞘流PESI（ad-sfPESI）。

Probe Electrospray Ionization (PESI) and Its Modified Versions: Dipping PESI (dPESI), Sheath-Flow PESI (sfPESI) and Adjustable sfPESI (ad-sfPESI).

作者信息

Hiraoka Kenzo, Ariyada Osamu, Usmanov Dilshadbek T, Chen Lee C, Ninomiya Satoshi, Yoshimura Kentaro, Takeda Sen, Yu Zhang, Mandal Mridul K, Wada Hiroshi, Rankin-Turner Stephanie, Nonami Hiroshi

机构信息

Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.

ARIOS INC., 3-2-20 Musashino, Akishima, Tokyo 196-0021, Japan.

出版信息

Mass Spectrom (Tokyo). 2020;9(1):A0092. doi: 10.5702/massspectrometry.A0092. Epub 2020 Dec 4.

DOI:10.5702/massspectrometry.A0092
PMID:33299735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7708747/
Abstract

In 2007, probe electrospray ionization/mass spectrometry (PESI/MS) was developed. In this technique, the needle is moved down along a vertical axis and the tip of the needle touched to the sample. After capturing the sample at the needle tip, the needle is then moved up and a high voltage is applied to the needle at the highest position to generate electrospray. Due to the discontinuous sampling followed by the generation of spontaneous electrospray, sequential and exhaustive electrospray takes place depending on the surface activity of the analytes. As modified versions of PESI, dipping PESI (dPESI), sheath-flow PESI (sfPESI) and adjustable sfPESI (ad-sfPESI) have been developed. These methods are complementary to each other and they can be applicable to surface and bulk analysis of various biological samples. In this article, the characteristics of these methods and their applications to real samples will be reviewed.

摘要

2007年，探针电喷雾电离/质谱（PESI/MS）技术得以开发。在该技术中，针头沿垂直轴向下移动，针头尖端接触样品。在针头尖端捕获样品后，针头向上移动，并在最高位置向针头施加高电压以产生电喷雾。由于采用不连续采样并随后产生自发电喷雾，根据分析物的表面活性会发生连续且彻底的电喷雾。作为PESI的改进版本，浸入式PESI（dPESI）、鞘流PESI（sfPESI）和可调鞘流PESI（ad-sfPESI）已被开发出来。这些方法相互补充，可应用于各种生物样品的表面和本体分析。在本文中，将对这些方法的特点及其在实际样品中的应用进行综述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/b7e808ccfb22/massspectrometry-9-1-A0092-figure18.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/9847c9aa0388/massspectrometry-9-1-A0092-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/23d9f0e1df7e/massspectrometry-9-1-A0092-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/ed24692a5241/massspectrometry-9-1-A0092-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/e60483e35b77/massspectrometry-9-1-A0092-figure04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/1f17e4478f65/massspectrometry-9-1-A0092-figure05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/4417b4aaf6bc/massspectrometry-9-1-A0092-figure06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/75057d7a3e38/massspectrometry-9-1-A0092-figure07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/04b9791b4902/massspectrometry-9-1-A0092-figure08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/65e618025b89/massspectrometry-9-1-A0092-figure09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/f62aab31c90f/massspectrometry-9-1-A0092-figure10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/bbb266ec7618/massspectrometry-9-1-A0092-figure11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/11154cd20a85/massspectrometry-9-1-A0092-figure12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/db1d49f972d3/massspectrometry-9-1-A0092-figure13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/1c607a659cd4/massspectrometry-9-1-A0092-figure14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/4172c4a914e0/massspectrometry-9-1-A0092-figure15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/e94b9d89c4e6/massspectrometry-9-1-A0092-figure16.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/75525a8b417d/massspectrometry-9-1-A0092-figure17.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/b7e808ccfb22/massspectrometry-9-1-A0092-figure18.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/9847c9aa0388/massspectrometry-9-1-A0092-figure01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/23d9f0e1df7e/massspectrometry-9-1-A0092-figure02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/ed24692a5241/massspectrometry-9-1-A0092-figure03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/e60483e35b77/massspectrometry-9-1-A0092-figure04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/1f17e4478f65/massspectrometry-9-1-A0092-figure05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/4417b4aaf6bc/massspectrometry-9-1-A0092-figure06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/75057d7a3e38/massspectrometry-9-1-A0092-figure07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/04b9791b4902/massspectrometry-9-1-A0092-figure08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/65e618025b89/massspectrometry-9-1-A0092-figure09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/f62aab31c90f/massspectrometry-9-1-A0092-figure10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/bbb266ec7618/massspectrometry-9-1-A0092-figure11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/11154cd20a85/massspectrometry-9-1-A0092-figure12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/db1d49f972d3/massspectrometry-9-1-A0092-figure13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/1c607a659cd4/massspectrometry-9-1-A0092-figure14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/4172c4a914e0/massspectrometry-9-1-A0092-figure15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/e94b9d89c4e6/massspectrometry-9-1-A0092-figure16.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/75525a8b417d/massspectrometry-9-1-A0092-figure17.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e49/7708747/b7e808ccfb22/massspectrometry-9-1-A0092-figure18.jpg

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