Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Am J Physiol Regul Integr Comp Physiol. 2012 Jan 1;302(1):R68-74. doi: 10.1152/ajpregu.00340.2011. Epub 2011 Oct 19.
The mouse has many advantages over other experimental models for the molecular investigation of left ventricular (LV) function. Accordingly, there is a keen interest in, as well as an intense need for, a conscious, chronically instrumented, freely moving mouse model for the determination of cardiac function. To address this need, we used a telemetry device for repeated measurements of LV function in conscious mice at rest and during exercise. For reference, we compared the responses in mice to the responses in identically instrumented conscious rats. The transmitter body of the telemetry device (rat PA-C40; mouse PA-C10; Data Sciences International, St. Paul, MN) was placed in the intraperitoneal space through a ventral abdominal approach (rat) or subcutaneously on the left flank (mouse). The pressure sensor, located within the tip of a catheter, was inserted into the left ventricle through an apical stab wound (18 gauge for rat; 21 gauge for mouse) for continuous, nontethered, recordings of pulsatile LV pressure. A minimum of 1 wk was allowed for recovery and for the animals to regain their presurgical weight. During the recovery period, the animals were handled, weighed, and acclimatized to the laboratory, treadmill, and investigators. Subsequently, LV parameters were recorded at rest and during a graded exercise test. The results document, for the first time, serial assessment of ventricular function during exercise in conscious mice and rats. This methodology may be adopted for advancing the concepts and ideas that drive cardiovascular research.
在对左心室 (LV) 功能进行分子研究方面，老鼠比其他实验模型具有许多优势。因此，人们对用于确定心脏功能的清醒、长期仪器化、自由活动的老鼠模型有着浓厚的兴趣和强烈的需求。为了满足这一需求，我们使用遥测设备在休息和运动期间重复测量清醒小鼠的 LV 功能。作为参考，我们比较了老鼠和相同仪器化的清醒大鼠的反应。遥测设备的发射体（大鼠 PA-C40；小鼠 PA-C10；Data Sciences International，明尼苏达州圣保罗）通过腹部腹侧入路（大鼠）或左侧腰部皮下（小鼠）放置在腹腔内。压力传感器位于导管尖端内，通过心尖刺伤（大鼠 18 号；小鼠 21 号）插入左心室，以连续、无束缚地记录搏动性 LV 压力。至少需要 1 周的时间让动物恢复和恢复到术前体重。在恢复期间，对动物进行处理、称重，并使其适应实验室、跑步机和研究者。随后，在休息和分级运动试验期间记录 LV 参数。这些结果首次记录了在清醒的老鼠和大鼠中在运动期间的心室功能的连续评估。该方法可用于推进推动心血管研究的概念和想法。
